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Transformation Lab. Engineering a Bacteria to Glow Green. Goal:. To get E. coli to take in a plasmid that contains the GFP gene and produce green fluorescence protein when given the right environmental signal. Plasmid. Contains : Origin of Replication Antibiotic Resistance

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transformation lab

Transformation Lab

Engineering a Bacteria to Glow Green

slide2
Goal:
  • To get E. coli to take in a plasmid that contains the GFP gene and produce green fluorescence protein when given the right environmental signal.
plasmid
Plasmid
  • Contains:
    • Origin of Replication
    • Antibiotic Resistance
    • Restriction site to enter gene
    • Operon to control turning on/off of gene
at the biotech company
At the biotech company…
  • Restriction enzyme
  • DNA ligase
what you will do
What you will do…
  • Heat shock
  • Culture bacteria in LB
  • Plate bacteria onto 4 plates:
    • LB
    • LB/amp
    • LB/amp
    • LB/amp/ara
will we see glowing bacteria on all our plates
Will we see glowing bacteria on all our plates?
  • Operon: Control switch that turns on/off conversion of DNARNAProtein
  • Arabinose operon: turns on the production of GFP when arabinose (sugar) is present as a signal.
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