Transformation with Firefly gene Lab Protocol. Procedure. The Plasmid pBestluc and the CaCl must be kept on ice throughout the entire experiment. Obtain the plate of Luria agar with the actively growing E. coli lawn on it.
and the CaCl must be kept on ice
throughout the entire experiment.
actively growing E. coli lawn on it.
of ice cold CaCl to the tube
10. Using forceps or wearing gloves place a sheet of nitrocellulose paper on the surface of the agar.
11. Lift the lid only enough to place the Nitrocellulose paper on the agar and replace the lid ASAP
13. After 15 min on ice, remove the tube and immediately place in a 42oC water bath for 75-90 seconds.
** Repeat step 18 with your LB Agar Plate (w/o Ampicillin). DO NOT USE NITROCELLULOSE PAPER FOR THIS DISH)
inverted - overnight at 37oC.