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Production of Bio-Hydrogen by Butanoic Acid Fermentation

Erasmus+. Sustainability by Biotechnology. Production of Bio-Hydrogen by Butanoic Acid Fermentation. Adrian Wawruschka. Jana Grombirikova. What is Biohydrogen?. Biohydrogen is molecular hydrogen gas ( produced in a biochemical way.

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Production of Bio-Hydrogen by Butanoic Acid Fermentation

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  1. Erasmus+ Sustainabilityby Biotechnology Productionof Bio-Hydrogen byButanoic Acid Fermentation Adrian Wawruschka Jana Grombirikova

  2. Whatis Biohydrogen? • Biohydrogen ismolecular hydrogen gas (produced in a biochemicalway. • Biohydrogen is a frequent productofmicrobialmetabolism, in particulartypesoffermentation.

  3. Fermentation • Degradation oforganicsubstances in anaerobicconditions • Energy isproducedbyparticularwaysoffermentation (substrate-level phosphorylation). Thatmeansthedirectphosphorylationfrom ADP underanaerobiccondition For e.g. Duringtheproductionofpyruvate and ATP out ofphosphoenolpyruvate and ADP duringglycolysis. ADP ATP Phosphoenolpyruvate Pyruvate

  4. Goal of Fermentation → Regeneration of NAD+, whichisusedduringglycolysis Nicotinamid Purin-Base Adenin 2 Phosphate-groups sugarRibose sugarRibose

  5. Goal ofthe Fermentation NAD+ NADH+H+ Built during fermentation Built during glycolysis

  6. 1. Step: Hydrolysis of Saccharose Disaccharide saccharoseishydrolyticallysplit Glucose and Fructose develop Glucose isoxidizedduringglycolysis Glycolysis

  7. Glycolysis Glucose Problem: Glycolysis consumed NAD+ In the nextstep, (butanoicfermentation), NAD+ will be regenerated again. 2 Glyceraldehyde-3-phosphate (3-PGA) 2 Pi, 2 NAD+ 2 NADH + H+ 2 2 Butanoic acid fermentation Pyruvate 1,3-Bisphosphoglycerate

  8. Butanoic Acid Fermentation 2 • During the first step of fermentation a total of 4and 4 electrons are realeased. • Ferredoxin accepts the electrons and passes them on to Hydrogen, while being catalyzed by Hydrogenase • Molecular Hydrogen is formed Ferredoxine Pyruvate 2 CoA-SH 2 2 2 2 Acetyl-CoA Acetyl-CoA CoA-SH ATP ADP+P NADH+ NAD+ NAD+ NADH+ Acetoacetyl-CoA 3-Hydroxybutyryl-CoA Butyryl-CoA Butyrate

  9. Closer Look at the First Step 2 CO2 2 Pyruvate 2 CoA e Ferredoxine 4 4 2 Acetyl-CoA 2 H2

  10. Butanoic Acid Fermentation 2 • During the first step of fermentation a total of 4and 4 electrons are realeased. • Ferredoxin accepts the electrons and passes them on to Hydrogen, while being catalyzed by Hydrogenase • Molecular Hydrogen is formed Ferredoxine Pyruvate 2 CoA-SH 2 2 2 2 Acetyl-CoA Acetyl-CoA CoA-SH ATP ADP+P NADH+ NAD+ NAD+ NADH+ Acetoacetyl-CoA 3-Hydroxybutyryl-CoA Butyryl-CoA Butyrate

  11. Butanoic Acid Fermentation Alcoholic Fermentation Glucose NAD+ NAD+ ADP ADP NADH + H+ ATP NADH + H+ H2 ATP Ethanal Acetyl-CoA ADP NAD+ ATP NAD+ Ethanol Butanoicacid

  12. Bacteria ofButanoic Acid Fermentation • SomebacteriaoftheClostridiumclass, e.g. Clostridiumacetobutylicum • These bacteria are saccharolytic, therefore they use saccharose as substrate( e.g. fromsugarbeetcuttings) • Condition of a neutral pH-value

  13. Summary: Butanioc Acid Fermentation • Overview over the overall reaction (stoichiometrically not correct): Glucose + 3 ADP + 3 Pi→ butanoicacid + 2 H2 + 2 CO2 + 3 ATP

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