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The application of DNA technology in Animal Forensic and Parentage Determination

The application of DNA technology in Animal Forensic and Parentage Determination. Presented by: Ms. N. Mapholi (MSc in Genetics). Animal forensics in livestock and game poaching Cattle and horse DNA profiling and Parentage Determination Species Identification on meat and process meat

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The application of DNA technology in Animal Forensic and Parentage Determination

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  1. The application of DNA technology in Animal Forensic and Parentage Determination Presented by: Ms. N. Mapholi (MSc in Genetics)

  2. Animal forensics in livestock and game poaching Cattle and horse DNA profiling and Parentage Determination Species Identification on meat and process meat Genetic Characterization Genetic Defects tests LIDCAT system Gene mapping and linkage studies ARC-API: Animal Genetics Lab

  3. Technologies • Genotyping • Microsatellites marker panels • Species specifics panels • Real time PCR • Primer designed kits • SNPs assays

  4. Stock theft and poaching Microsatellites markers Link the crime scene and the suspect DNA evidences obtained Parentage dispute cases Different marker panels Bovine, Horse, Ovine, Goat, Porcine, Buffalo, Impala, Kudu, Wildebeest, Eland, Sable, Rhino and Blesbok FORENSIC WORK

  5. Chain of custody Herd of sheep Crime scene New case 3130xL Gene scan Thermal cycler DNA extract DNA Samples

  6. Court Results Interpretation DNA Report Jail Judgement

  7. DNA samples from both parents and calf Alleged mothers and dispute calf It is important to take note that in the absence of either parent (bull or cow) paternity cannot be proven. Only when both parents are analyzed it can be proven beyond doubt that the parents are the real biological parents of the offspring in question Parentage dispute

  8. Parentage dispute Cow 1 Cow 2 Inclusion/ exclusion as possible mothers Calf 1?

  9. … Procedure of sampling hair Hair Samples PCR tubes PCR tray Genetic analyzer PCR machine

  10. Service rendered to the breed societies and stud breeders for bovine and horses DNA profiling certificates are issued Parentage determination for breeding and selection purpose Buffalo DNA profiling – this is new and still building the database DNA profiling

  11. DNA based technology Species identification on meat and processed meat products biltong, wors, patties, sausages, mince meat, Vienna Species specific primer designed kits SNP Assays for genetic defects tests in bovine and porcine Dexter chondodysplasia and Malignant hyperthermia Real Time PCR

  12. Most of livestock species haven been characterized in our lab using microsatellites markers Several multiplexes have been developed and optimized in our lab Genetic characterization

  13. LIDCAT DATABASE • Lidcat package – for tail hair collection

  14. Information needed on LIDCAT system • Animal ID (ear tag number) • Species (cattle) • Breed (Nguni) • Gender (female or male) • Name of the owner (Olivia Mapholi) • Postal address of owner • Contact numbers

  15. Ms. Olivia Mapholi Tel : 012 672 9027/ 9008/ 9187 Fax: 012 672 9214 Email: ntanga@arc.agric.za jill@arc.agric.za Contact details

  16. Thank you Any questions?

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