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DNA 重組成功的篩選方法 - 菌落藍白篩選

DNA 重組成功的篩選方法 - 菌落藍白篩選. 確認載體成功進入細菌 使用抗生素標記 確認基因有接入載體 lacZ 基因被插壞. 乳糖操作組. 半乳糖苷酶 滲入酶 乙醯基轉化酶. 無作用之 抑制劑. 誘導物. (Klein et al.2003). 確定 DNA 重組成功的方法. LacZ 基因可產生半乳糖苷酶，分解 X-gal 生成藍色菌落 LacZ 基因被外來 DNA 插壞，不產生半乳糖苷酶，無法分解 X-gal ，菌落呈白色. 半乳糖苷酶.

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DNA 重組成功的篩選方法 - 菌落藍白篩選

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  1. DNA重組成功的篩選方法-菌落藍白篩選 • 確認載體成功進入細菌 • 使用抗生素標記 • 確認基因有接入載體 • lacZ基因被插壞

  2. 乳糖操作組 半乳糖苷酶 滲入酶 乙醯基轉化酶 無作用之 抑制劑 誘導物 (Klein et al.2003)

  3. 確定DNA重組成功的方法 • LacZ基因可產生半乳糖苷酶，分解X-gal生成藍色菌落 • LacZ基因被外來DNA插壞，不產生半乳糖苷酶，無法分解X-gal，菌落呈白色

  4. 半乳糖苷酶 X-gal (5-bromo-4-chloro-3-indoyl-β-D-galactopyranoside )

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