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MENA3100, 14.01.09 OBK. Optical microscopy. Magnification Resolution Contrast. We want to see more details. Simple microscope. The simple microscope. Antony van Leeuwenhoek. (1632−1723). The compound microscope. ca. 1595 Hans Janssen. Robert Hook (1635−1703). Micrographia , 1664.

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slide1

MENA3100, 14.01.09

OBK

Optical microscopy

Magnification

Resolution

Contrast

We want to see more details

Simple microscope

slide2

The simple microscope

Antony van Leeuwenhoek

(1632−1723)

slide3

The compound microscope

ca. 1595 Hans Janssen

Robert Hook

(1635−1703)

Micrographia, 1664

slide6

Compound microscope

for transmitted light

slide7

Inverted compound microscope

for reflected light

slide9

Refraction

Kromatisk abberation

slide11

The objective

Corrections

slide12

Numerical aperture (NA)

NA = nsina

Resolution

R = 0,61l/NA

Rayleigh criterion

(Raleigh in Brandon and Kaplan)

slide13

The limiting factor: Diffraction

NA = nsina

Oil immersion

slide15

More is needed than just the objective

Condenser system

Eyepice

Ocular (okular)

slide19

Amorphous and cubic: Optically isotropic, one refracive index

Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices

Lower symmetry: Optically anisotropic, three refractive indices

slide24

Remember that geologists often call their book on microscopy on thin sections for

optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi).

Some web sites:

http://www.olympusmicro.com/primer/

http://www.microscopyu.com/

http://www.micro.magnet.fsu.edu/

http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf

maintenance:

http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf

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