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MENA3100, 14.01.09 OBK. Optical microscopy. Magnification Resolution Contrast. We want to see more details. Simple microscope. The simple microscope. Antony van Leeuwenhoek. (1632−1723). The compound microscope. ca. 1595 Hans Janssen. Robert Hook (1635−1703). Micrographia , 1664.

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MENA3100, 14.01.09

OBK

Optical microscopy

Magnification

Resolution

Contrast

We want to see more details

Simple microscope


The simple microscope

Antony van Leeuwenhoek

(1632−1723)


The compound microscope

ca. 1595 Hans Janssen

Robert Hook

(1635−1703)

Micrographia, 1664



Compound microscope

for transmitted light


Inverted compound microscope

for reflected light


Refraction

Kromatisk abberation



The objective

Corrections


Numerical aperture (NA)

NA = nsina

Resolution

R = 0,61l/NA

Rayleigh criterion

(Raleigh in Brandon and Kaplan)


The limiting factor: Diffraction

NA = nsina

Oil immersion


More is needed than just the objective

Condenser system

Eyepice

Ocular (okular)



Amorphous and cubic: Optically isotropic, one refracive index

Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices

Lower symmetry: Optically anisotropic, three refractive indices





Remember that geologists often call their book on microscopy on thin sections for

optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi).

Some web sites:

http://www.olympusmicro.com/primer/

http://www.microscopyu.com/

http://www.micro.magnet.fsu.edu/

http://www.micro.magnet.fsu.edu/primer/pdfs/basicsandbeyond.pdf

maintenance:

http://www.zeiss.de/C1256D18002CC306/0/1205E8CBD68054EAC125701500404705/$file/46-0009_e.pdf


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