Cortical plaques
This presentation is the property of its rightful owner.
Sponsored Links
1 / 12

Cortical plaques PowerPoint PPT Presentation


  • 68 Views
  • Uploaded on
  • Presentation posted in: General

Cortical plaques. Lambotin et al , J Cell Science 2005. Ezrin. Actine (nid d’abeille). GTPases. Doulet et al , J Cell Biol 2006. PI3K. Nécessaire à l’adhésion "forte" en condition de flux (G. Mikaty, G. Dumenil & coll). Lambotin et al , J Cell Science 2005. β -arrestins. A. B.

Download Presentation

Cortical plaques

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Cortical plaques

Cortical plaques

Lambotin et al, J Cell Science 2005

Ezrin

Actine

(nid d’abeille)

GTPases

Doulet et al,

J Cell Biol 2006

PI3K

Nécessaire à l’adhésion "forte" en condition de flux

(G. Mikaty, G. Dumenil & coll)

Lambotin et al, J Cell Science 2005


Cortical plaques

β-arrestins

A

B

C

  • MapK

  • Src

  • Akt

Lan Ma and Gang Pei, Journal of Cell Science 120, 213-218 (2007)


Cortical plaques

β-arrestins - SiRNA

Beta arrestine 2

GFP

ezrin

βarrestin 1/2 siRNA

Ezrin recruitment

Expression is

reduced by

Control siRNA

100%

80%

βarrestin 1/2

2,5 fold

60%

Ezrin

Actin

40%

20%

0%

Control

Arrestin 1+2

FLNA sirRNA

GRK2 sirRNA

siRNA

sirRNA


Cortical plaques

β-arrestins - SiRNA

Beta arrestine 2

GFP

ezrin

βarrestin 1/2 siRNA

Ezrin recruitment

Expression is

reduced by

Filamin A siRNA

Control siRNA

GRK2 siRNA

100%

80%

βarrestin 1/2

2,5 fold

60%

Ezrin

Filamin A

7,5 fold

40%

GRK2

20%

3 fold

0%

Actin

Control

Arrestin 1+2

FLNA sirRNA

GRK2 sirRNA

siRNA

sirRNA


Cortical plaques

Ezrin recruitment

100

80

60

% of recruiting colony

40

20

0

Control

Phentolamine

10 uM

Propranolol

10 uM

Recepteur adrénergique

ezrin

Beta2 AR - YFP

α-antagonist

β-antagonist


Cortical plaques

Ezrin recruitment

100

80

60

% of recruiting colony

40

20

0

Ct

0,0001

0,01

1

10

Isoproterenol (μM)

β-agonist

Recepteur adrénergique

ezrin

Beta2 AR - YFP

+


Cortical plaques

+

Ezrin recruitment

100

80

60

% of recruiting colony

40

20

0

Ct

0,0001

0,01

1

10

Isoproterenol (μM)

β-agonist

Recepteur adrénergique

ezrin

Beta2 AR - YFP


Cortical plaques

Ezrin recruitment

Ezrin recruitment

100%

100%

80%

80%

60%

60%

40%

40%

20%

20%

0%

0%

IL8

MIP

Isoproterenol

Propranolol

  • 10µM 10µM 1µM 1µM - - + - +

AT2

SDF1

control

Isoproterenol 1uM

Isoproterenol 100nM

5 à10 x Kd

Recepteur adrénergique


Cortical plaques

Ezrin recruitment

100

80

60

% of recruiting colony

40

20

0

PKA-PS

Forkolin

10-5 M

PKA-PS

Forkolin

10-6 M

Control

20uM

10uM

Signaling des protéines G


Cortical plaques

Activation partielle


Cortical plaques

Billes coatées, cellules hCMEC/D3

contraste

actin

ezrin

Isoproterenol

β1 agoniste

Phenylephrine

α1 agoniste

BSA ou Metoprolol


Cortical plaques

Recrutment d’Ezrin

Recrutment d’Ezrin

100

100

80

80

60

60

% recruiting colonies

% recruiting colonies

40

40

20

20

0

0

Ct

0,0001

0,01

1

10

Ct

0,0001

0,01

1

100

Iso +

Isoproterenol (μM)

Phenylephrine (μM)

Phe

10:10

5

4

3

Ezrin phosphorylation

fold increase

2

1

0

Isoproterenol

Ct

Effet des agonistes adrénergiques

Isoproterenol

Ct

Nm - + - +

P-Ezrin

P-Moesin


  • Login