The Effects of Ampicillin on the Growth of E. Coli - PowerPoint PPT Presentation

The effects of ampicillin on the growth of e coli
Download
1 / 14

  • 281 Views
  • Uploaded on
  • Presentation posted in: General

The Effects of Ampicillin on the Growth of E. Coli. Michelle Anthony Khadija Lawrence . Escherichia coli . Gram-negative Rod shaped Pathogenic strains . http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg. Ampicillin .

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.

Download Presentation

The Effects of Ampicillin on the Growth of E. Coli

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


The effects of ampicillin on the growth of e coli

The Effects of Ampicillin on the Growth of E. Coli

Michelle Anthony

Khadija Lawrence


Escherichia coli

Escherichiacoli

  • Gram-negative

  • Rod shaped

  • Pathogenic strains

http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg


Ampicillin

Ampicillin

http://upload.wikimedia.org/wikipedia/commons/4/48/Ampicillin-2D-skeletal.png


Hypothesis

Hypothesis

  • The maximum growth of E. coli will be inhibited as the concentration of ampicillin is increased.


Components

Components

  • Culture (E. coli)

  • Ampicillin

  • 10x lower LB

  • H2O


Experiments 1 4

Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 1 41

Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 1 42

Experiments 1-4

  • P. fluorescens

    • resistant

  • Titrations

  • No derived conclusion

  • Lead to redesign


Experiments 5 10

Experiments 5-10

  • No titrations

    • Altered amounts of ampicillin

  • Varied the ranges each week


Experiments 5 101

Experiments 5-10

  • No titrations

    • Altered amounts of ampicillin

  • Varied the ranges each week

  • Results from each week lead to

    final experiment


Experimental design

Experimental Design

* All values in table are in microL


The effects of ampicillin on the growth of e coli

3

0

5

9

12

16


Conclusion derived from results

Conclusion derived from Results

  • Maximum growth was not inhibited

    • Normal curve max: 1.088

    • Highest amp max: 1.044

  • Lag phase dramatically affected after 16microL is exceeded

  • IC50 not determined

  • Ampicillin affects length of lag phase

    • Graph consistent with scale


References

References

  • http://www.cdc.gov/ecoli/general/

  • http://www.foodsafety.gov/poisoning/causes/bacteriaviruses/ecoli/

  • http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1583696/?page=4


  • Login