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Voltage Clamp PowerPoint PPT Presentation


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Voltage Clamp. Patch Clamp Applications. Evidence that potassium is primarily responsible for the RMP. Neuron membrane is at rest. Neuron membrane is at rest. Now let’s look at the responses of a neuron to various stimuli.

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Voltage Clamp

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Voltage clamp l.jpg

Voltage Clamp


Patch clamp applications l.jpg

Patch Clamp Applications


Slide3 l.jpg

  • Evidence that potassium is primarily responsible for the RMP.


Neuron membrane is at rest l.jpg

Neuron membrane is at rest


Neuron membrane is at rest5 l.jpg

Neuron membrane is at rest


Now let s look at the responses of a neuron to various stimuli l.jpg

Now let’s look at the responses of a neuron to various stimuli


Slide11 l.jpg

  • Evidence that sodium is responsible for inward current during depolarization phase of the action potential. Use of the voltage clamp to measure current.


Normal membrane current during the action potential l.jpg

Normal membrane current during the action potential.


Membrane current when there is no na contribution l.jpg

Membrane current when there is no Na contribution.


Combine these two curves l.jpg

Combine these two curves


The bottom curve is a subtraction of the other two showing the effect of sodium current l.jpg

The bottom curve is a subtraction of the other two, showing the effect of sodium current.


Slide16 l.jpg

  • Another experiment demonstrates the same conclusion, again using the voltage clamp.


Slide17 l.jpg

These curves are all “normal” curves during action potential, with the membrane held at various voltages by the voltage clamp.


E na 50 mv in this experiment l.jpg

ENa+ ~ 50 mV in this experiment


This diagram is similar to fig 5 21 l.jpg

This diagram is similar to Fig. 5.21


Slide22 l.jpg

  • The remainder of this presentation will focus on the actions and effects of synapses


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