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INTERNSHIP PRESENTATION. Aman ullah zafar 2010-ag-3581 Soil Bacteriology Section Ayub Agriculture Research institute (AARI), Faisalabad. Work that I have done.

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INTERNSHIP PRESENTATION

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Internship presentation

INTERNSHIP PRESENTATION

Amanullahzafar

2010-ag-3581

Soil Bacteriology Section

Ayub Agriculture Research institute (AARI),

Faisalabad


Internship presentation

Work that I have done

  • Case study; Role of different precursor’s and PGPR inoculation on growth of maize.

  • Media preparation and isolations

  • Soil analysis


Case study role of different precursor s and pgpr inoculation on growth of maize

CASE STUDYROLE OF DIFFERENT PRECURSOR’S AND PGPR INOCULATION ON GROWTH OF MAIZE.


Introduction

INTRODUCTION

Maize (Zea mays L.).

3rd most important crop after wheat and rice.

Use as both grain and fodder.

High fertilizer demand and huge population converted interest to use biofertilizers.

Biofertilizers are eco-friendly, inexpensive and produce resistance to plants. (Mehboobet al., 2008)


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L-tryptophan (precursor of auxin)

Application may increase growth of plants (Zahiret al., 2010).

L-Tryptophan (L-TRP) involve in microbial biosynthesis of auxin.

Exogenously application produce higher values of auxins and exert positive effect. (Khalid et al., 2004)


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L-adenine (precursor of cytokinins)

Cytokinins affect growth by cell division, morphogenesis, lateral buds and stomatal opening.

Exogenous application enhances

growth and yield of crops.

Stimulate nitrate reductase activity. (Rao et al., 2013).

Glutamate synthetase

Spikelet number per tassel

Increase chlorophyll and protein content. (Below and Gentry., 2002)


Azotobacter

Azotobacter

The genus Azotobacter is comprised of bacteria that require the presence of oxygen to grow and reproduce,

Azotobacter has the ability to "fix" atmospheric nitrogen.

Inoculation of precursors with PGPR increases growth and yield of crops. (Ciccilloet al., 2002).

Present study was planned to assess the beneficial effect of PGPR with precursor of auxin (L-tryptophan) and cytokinin (L-adenine) on growth of maize.


Methodology

METHODOLOGY

Isolation and purification of Azotobactersp

Preparation of Jensen’s Medium


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Isolation from soil sample by dilution method and plating of medium.

Sterilization of medium.

1g soil + 99 mL of sterilize water for dilutions (10-2, 10-4, 10-6).

streaking on Petri plants by inoculating needle.

Incubation for 48 hr at 25oC.

Single colonies were picked and purified on fresh plates.

Purified colonies stored at 5±1oC on slants and maintained for further exp.


Preparation of inoculum

Preparation of inoculum

Jensen broth prepared and autoclaved at 121oC and15 psi

Broth inoculated with purified and selected isolates of Azotobacter sp

Incubated at 25oC for 3 days


Pot study

Pot study

Complete Randomized Design (CRD) with six treatments and three replications.

Treatments

T1=Control

T2=L-Tryptophan

T3=L-Adenine

T4 =Azotobacter sp

T5 = L-Tryptophan +Azotobacter sp

T6 = L-Adenine +Azotobacter sp


Results

Results

Effect of treatments on root and shoot parameters of maize.


Conclusion

conclusion

Precursors i.e. L-tryptophan and L-adenine and PGPR inoculation alone have improved the growth of maize.

The growth promotion is much improved when the precursors i.e. L-tryptophan and L-adenine are applied with PGPR inoculation.

Hence, precursor-inoculum interaction has more pronounced effect on the growth of maize.


Literature cited

LITERATURE CITED

Below and Gentry., 2002. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Research J. Agricultural and Biological Sciences, 1: 210-215.

Mehboob and S. Kumar 2008. Potential of Azotobacter and Azospirillum as biofertilizers for upland agriculture: A re view. J. Sci. Ind. Res. 48:134 -144. [Field Crop Abst. 42; 10066; 2008].

Rao, S. R., A. Qayyum, A. Razzaq, M. Ahmad, I. Mahmood and A. Sher (2013). Role of foliar application of salicylic acid and L-tryptophan in drought tolerance of maize. The J. Anim. and Plant Sciences, 22(3): 768-772.

Zahir, Z.A., H.N. Asghar, M.J. Akhtar and M. Arshad (2010). Precursor (L-tryptophan) inoculum (Azotobacter) interactions for improving yields and nitrogen uptake of maize. J. Plant Nutrition 28: 805-817.

Ciccillo, F., A. Fiore, A. Bevivino, C. Dalmastri, S. Tabacchioni and L. Chiarini(2002). Effects of two different application methods of Burkholderiaambifaria MCI 7 on plant growth and rhizospheric bacterial diversity. J. EnvironmentalMicrobiology, 4(4), 238–245.

Khalid, A., M. Arshad and Z.A. Zahir (2004). Screening plant growth promoting rhizobacteria for improving growth and yield of wheat. J. Appl. Microbiol., 96: 473-480.


Microbes isolated in lab

MICROBES ISOLATED IN LAB

Free living

Azotobacter

Azospirillum

Phosphorus Solubilizers

Bacillus

Pseudomonas

Symbiotic

Rhizobium (Nitrogen fixer)


Isolation of rhizobium

ISOLATION OF RHIZOBIUM

Pink Healthy Nodules Washing Sterilization (95% Ehanol) Washing 0.1% HgCl2

Washing Crushing Streaking on YMA Petri Plate


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Fig: Isolation of Rhizobium on Yeast Minnitol Agar Media


Isolation of free livings dilution plate technique

ISOLATION OF FREE LIVINGS Dilution Plate Technique


Fig isolation of azotobacter

Fig: Isolation of Azotobacter


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Fig: Isolation of Azospirillum


Results1

Results


Soil analysis

SOIL ANALYSIS

  • Soil samples were analyzed for:

  • Phosphorus (Olsen and Sommers method)

  • Organic matter (walkley black method)


Results table determination of available phosphorous from different soil sample

ResultsTable: Determination of available Phosphorous from different soil sample


Results table determination of available organic matter from different soil sample

ResultsTable: Determination of available organic matter from different soil sample


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