INTERNSHIP PRESENTATION. Aman ullah zafar 2010-ag-3581 Soil Bacteriology Section Ayub Agriculture Research institute (AARI), Faisalabad. Work that I have done.
Soil Bacteriology Section
Ayub Agriculture Research institute (AARI),
Maize (Zea mays L.).
3rd most important crop after wheat and rice.
Use as both grain and fodder.
High fertilizer demand and huge population converted interest to use biofertilizers.
Biofertilizers are eco-friendly, inexpensive and produce resistance to plants. (Mehboobet al., 2008)
L-tryptophan (precursor of auxin)
Application may increase growth of plants (Zahiret al., 2010).
L-Tryptophan (L-TRP) involve in microbial biosynthesis of auxin.
Exogenously application produce higher values of auxins and exert positive effect. (Khalid et al., 2004)
L-adenine (precursor of cytokinins)
Cytokinins affect growth by cell division, morphogenesis, lateral buds and stomatal opening.
Exogenous application enhances
growth and yield of crops.
Stimulate nitrate reductase activity. (Rao et al., 2013).
Spikelet number per tassel
Increase chlorophyll and protein content. (Below and Gentry., 2002)
The genus Azotobacter is comprised of bacteria that require the presence of oxygen to grow and reproduce,
Azotobacter has the ability to "fix" atmospheric nitrogen.
Inoculation of precursors with PGPR increases growth and yield of crops. (Ciccilloet al., 2002).
Present study was planned to assess the beneficial effect of PGPR with precursor of auxin (L-tryptophan) and cytokinin (L-adenine) on growth of maize.
Isolation and purification of Azotobactersp
Preparation of Jensen’s Medium
Sterilization of medium.
1g soil + 99 mL of sterilize water for dilutions (10-2, 10-4, 10-6).
streaking on Petri plants by inoculating needle.
Incubation for 48 hr at 25oC.
Single colonies were picked and purified on fresh plates.
Purified colonies stored at 5±1oC on slants and maintained for further exp.
Jensen broth prepared and autoclaved at 121oC and15 psi
Broth inoculated with purified and selected isolates of Azotobacter sp
Incubated at 25oC for 3 days
Complete Randomized Design (CRD) with six treatments and three replications.
T4 =Azotobacter sp
T5 = L-Tryptophan +Azotobacter sp
T6 = L-Adenine +Azotobacter sp
Effect of treatments on root and shoot parameters of maize.
Precursors i.e. L-tryptophan and L-adenine and PGPR inoculation alone have improved the growth of maize.
The growth promotion is much improved when the precursors i.e. L-tryptophan and L-adenine are applied with PGPR inoculation.
Hence, precursor-inoculum interaction has more pronounced effect on the growth of maize.
Below and Gentry., 2002. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Research J. Agricultural and Biological Sciences, 1: 210-215.
Mehboob and S. Kumar 2008. Potential of Azotobacter and Azospirillum as biofertilizers for upland agriculture: A re view. J. Sci. Ind. Res. 48:134 -144. [Field Crop Abst. 42; 10066; 2008].
Rao, S. R., A. Qayyum, A. Razzaq, M. Ahmad, I. Mahmood and A. Sher (2013). Role of foliar application of salicylic acid and L-tryptophan in drought tolerance of maize. The J. Anim. and Plant Sciences, 22(3): 768-772.
Zahir, Z.A., H.N. Asghar, M.J. Akhtar and M. Arshad (2010). Precursor (L-tryptophan) inoculum (Azotobacter) interactions for improving yields and nitrogen uptake of maize. J. Plant Nutrition 28: 805-817.
Ciccillo, F., A. Fiore, A. Bevivino, C. Dalmastri, S. Tabacchioni and L. Chiarini(2002). Effects of two different application methods of Burkholderiaambifaria MCI 7 on plant growth and rhizospheric bacterial diversity. J. EnvironmentalMicrobiology, 4(4), 238–245.
Khalid, A., M. Arshad and Z.A. Zahir (2004). Screening plant growth promoting rhizobacteria for improving growth and yield of wheat. J. Appl. Microbiol., 96: 473-480.
Rhizobium (Nitrogen fixer)
Pink Healthy Nodules Washing Sterilization (95% Ehanol) Washing 0.1% HgCl2
Washing Crushing Streaking on YMA Petri Plate
Fig: Isolation of Rhizobium on Yeast mediumMinnitol Agar Media
Fig: Isolation of mediumAzospirillum