Group 2 - The Enhancers Gene Expression

1. Group 2 - The EnhancersGene Expression Unit Learning Goal Identify the sequence elements of a gene and describe their functions A. Arrange the sequence elements of a gene in a functional order B. Predict the effect on gene expression if components of functional genes are deleted, inserted, or rearranged C. Apply these concepts to a transgenic model D. Integrate the functional relationships linking protein, RNA, and DNA

2. Group 2 - Gene Expression • Active Learning Activity The exercise proposed is an in-class activity utilizing “clickers”. This section will be preceded and will compliment the gene structure exercise elaborated by our group. Students are encouraged to break into small groups to discuss the questions posed to them.

3. Group 2 - Gene Expression • Introduction Short lecture to introduce the idea of transgenics, especially as it applies to the mouse system. The following slides will introduce the GFP gene in Jellyfish, its natural environment, and then we show this gene being introduced into a mouse – transgene.

4. Jellyfish have the GFP gene which produces a “green fluorescent protein”

5. Clicker Question 1: Why is this mouse green? • It is producing the Green Fluorescent Protein (GFP) b. It is transgenic for the GFP gene c. It is expressing the GFP gene d. All of the above e. None of the above

6. Assume that this mouse is entirely green.

7. Discuss tissue specific expression of the transgene. Enhancer Promoter Structural gene Reintroduce the modular nature of gene expression or gene Regulation. Regulation can be separated from the structural gene And that will emphasize where that protein is made is based on the enhancer, andwhich proteins is made depends on the structural Gene.

8. How do these two mice differ?

9. Clicker question 2:Why are only their ears, tails and toes green ? • Only the transgenic tissues fluoresce • Jellyfish genes can only be expressed in the skin of mice • c. Only skin cells contain the DNA regulatory sequences for expressing GFP • The GFP transgene linked to mouse regulatory sequences • These mice all ate jellyfish

10. In order for the GFP gene to function in the transgenic mouse? • the GFP gene must be similar in DNA sequence to a mouse gene • the GFP protein must be similar in protein sequence to a mouse protein • The GFP gene must be transcribed and translated • All of the above

11. If the mouse has a jellyfish gene, does that make it a jellyfish? • Yes • No