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CRC FISH/Cytogenetics

CRC FISH/Cytogenetics. Atlanta December 6, 2007. CRC FISH/Cytogenetics Team. Paola Dal Cin Brigham and Women's Marie Dell'Aquila UC San Diego Nyla Heerema Ohio State University Prasad Kodura Long Island Jewish Daniel Van Dyke Mayo Clinic

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CRC FISH/Cytogenetics

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  1. CRC FISH/Cytogenetics Atlanta December 6, 2007

  2. CRC FISH/Cytogenetics Team Paola Dal Cin Brigham and Women's Marie Dell'Aquila UC San Diego Nyla Heerema Ohio State University Prasad Kodura Long Island Jewish Daniel Van Dyke Mayo Clinic CRC Participants in Cytogenetics work: Laura Rassenti Tissue Core Director Andrew Greaves Bioinformatics Director Donna Neuberg Biostatistician Carlo Croce PI of Project 1

  3. CRC FISH Aims • Standardization of FISH Panel • Proficiency/consistency testing • Research plans: Genetics of CLL • Clinical significance of cytogenetic findings • Identify & test new loci

  4. First FISH Proficiency Exercise All of the normal results were below the normal cut-off

  5. Tissue Core Cytogenetic Datacompleteness ?? DFCI 65% LIJ 13% MC 57% MDA 71% OSU 65% UCSD 45%

  6. Aim 2. Role of miRNA genes (Croce)miR 15a & miR 16-1 tested probe too smallmiR 29 & miR 181, which regulate TCL1 MiR Clone # Locus miR-15a/16-1    RP11-240F24 & RP11-1008O15 miR-15b/16-2    RP11-436F13 & RP11-430E3 miR-29a/29b-1   RP11-10I12 & RP11-55L21 miR-29b-2/29c   RP11-656J8 & RP11-454L1 miR-181a-1/181b-1   RP11-106H7 & RP11-936I21 miR-181a-2/181b-2    RP11-1084I5 &RP11-348K2 miR-181c/181d    RP11-63F22

  7. Future studies • Concurrent FISH & CpG in early stage CLL; correlate with progression • >1 FISH detectable defect in a single CLL B-cell: incidence, risk parameters, progression • Does FISH abnormality influence CLL cell survival on stromal cells? • Does % cells with 11q- or 17p- influence clinical course or prognostic profile (<20%+ vs >50%+) • CGH microarray vs FISH vs CpG – which results best predict risk & progression?

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