1 / 18

Do They Eat Petroleum Hydrocarbons? Bioremediation of Toluene by Oil-Water Separator Bacteria

Do They Eat Petroleum Hydrocarbons? Bioremediation of Toluene by Oil-Water Separator Bacteria. LISA L. LOFTIS and JAMES E. CHAMPINE. Biology Department, Southeast Missouri State University. Site History. Former Gasoline Station Soil and Groundwater Contamination

makan
Download Presentation

Do They Eat Petroleum Hydrocarbons? Bioremediation of Toluene by Oil-Water Separator Bacteria

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Do They Eat Petroleum Hydrocarbons? Bioremediation of Toluene by Oil-Water Separator Bacteria LISA L. LOFTIS and JAMES E. CHAMPINE. Biology Department, SoutheastMissouri State University.

  2. Site History • Former Gasoline Station • Soil and Groundwater Contamination • Why Remediation System was chosen

  3. Site Map

  4. Soil Vapor Extraction Trailer

  5. Hypothesis • Contaminated groundwater represents a natural enrichment for petroleum hydrocarbon degrading bacteria

  6. Approach • Use toluene as a surrogate for petroleum related hydrocarbons that may be degraded in the aquifer • Test groundwater biofilm and pure cultures from the site for toluene degradation activity

  7. Site Materials • Before and After groundwater samples • Oil/Water Separator Biofilm (Jar and Vial) • Collection of Bacterial Isolates

  8. Gas Chromatograph

  9. Chromatography Conditions • Carrier Gas • DB1 30 m capillary column • 200ºC oven temperature • Flame Ionization Detector

  10. Typical chromatogram

  11. Serum Bottle Cultures • 20 mL of sterile strength 0.1x TSB • Teflon Rubber Stoppers • Aluminum Crimps • Amended with 10 L of Toluene • Aerobic

  12. Serum Bottle Cultures

  13. Before Groundwater Experiment

  14. After Groundwater Experiment

  15. Isolations • Before • Beige (from After groundwater) • ECP 6 • ECP 7

  16. Toluene Degraders ECP 6 and ECP 7

  17. Conclusions • Intrinsic Bioremediation did occur at the site • Site cleaned up in half of the estimated time • Bacteria with toluene degradation activity were isolated

  18. Acknowledgments • Southeast Missouri State University Undergraduate Research Program • Heather DuShell • Dr. Bjorn Olesen and the Department of Chemistry • Vicki Howell, Biology Dept. Secretary

More Related