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BIS- Mid

*. BIS- Mid. BSP primers. BIS-5 ’. BIS-3 ’. CT2. 75%. 70%. 64%. 70%. 52%. 88%. 69%. 75%. 68%. 70%. 65%. 62%. 72%. 67%. 73%. 60%. 59%. 66%. 52%. 62%. 55%. CT3. CT7. D4Z4. hhspm3. DUX4. LSau. A. CT9. CT-C-I1. CT-C-II2. CT-D-I2. Gaillard et al. figure e1.

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BIS- Mid

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  1. * BIS-Mid BSP primers BIS-5’ BIS-3’ CT2 75% 70% 64% 70% 52% 88% 69% 75% 68% 70% 65% 62% 72% 67% 73% 60% 59% 66% 52% 62% 55% CT3 CT7 D4Z4 hhspm3 DUX4 LSau A CT9 CT-C-I1 CT-C-II2 CT-D-I2 Gaillard et al. figure e1.

  2. Figure e-1: Methylation status across the D4Z4 repeat by sodium bisulfite sequencing in controls. For controls, 7 samples were analyzed. Samples CT-C-I1; CT-C-II2 are non-carrier non-affected members of family C and CT-D-I2 of family D (table e4). The position of the three different regions analyzed within D4Z4 is indicated above the corresponding column (from left to right, BIS-5’; Middle, BIS-Mid and right, BIS-3’). Each row of dots corresponds to a cloned DNA molecule. Black dots correspond to methylated CpG, white dots to unmethylatedCpGs and absence of dot represents sequence variation compared to the reference sequence. The percentage of methylated CpG among all CpGs/individual analyzed is given below each sample.

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