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Annotation Update

Annotation Update. By: Sandra, Tal, Morella. MarQuardt: A3 Phage Annotated. Genes 1-20 Tal: Genes 1-8 Morella: Genes 9-16 Sandra: Genes 17-20. Areas of Confusion. Is this gene’s coding potential all included?. More Areas of Confusion.

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Annotation Update

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  1. Annotation Update By: Sandra, Tal, Morella

  2. MarQuardt: A3 Phage Annotated • Genes 1-20 • Tal: Genes 1-8 • Morella: Genes 9-16 • Sandra: Genes 17-20

  3. Areas of Confusion • Is this gene’s coding potential all included?

  4. More Areas of Confusion • It was hard to see which gene was which between DNA Master and the Phamerator Maps • Gene 1 was confusing as to whether it was actually a gene or not because of all the factors that played in • Gene 1 was also difficult because it was the first gene annotated

  5. Genes Added or Deleted • No genes were added to our annotated genes • Gene 1 was deleted -Lack of start codon -No Blast match -Gene is too short -Coding potential is just a peak

  6. Interesting Features • There are 3 tRNAs in our section to annotate • We have not annotated them yet

  7. Interesting features • Gene 5 and 7 had an overlap with the previous gene, so they were both changed and their SD scores and ORFs were lowered Gene 5 Gene 7

  8. More Interesting Features • For the blast match, genes 10-16 match with Vix or Bxz2 • Only gene 9 matches with JHC117 in our sequence annotated • For the blast match, genes 17-20 all matched with Bxz2

  9. Phamerator Summary

  10. Gene 8 Pham Circle • This map displays that Pham 4284 is found in many different Clusters

  11. Map of Patience and MarQuardt

  12. Questions Remaining • Coding potential of gene 16 • Genes 17-20 were straightforward and only a few start codons were changed • The 3 tRNAs need to be annotated

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