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AP Lab Skills Guide

AP Lab Skills Guide. AP Lab Skills Guide. AP Lab Skills Guide. +. Mix contents Begin Timing. Tube 1 (substrate) Tube 2 (enzyme). Tube 3 (tubes 1 + 2) Begin Timing. H 2 O 2 Guaiacol Distilled H 2 O. Peroxidase Distilled water . AP Lab Skills Guide.

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AP Lab Skills Guide

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  1. AP Lab Skills Guide

  2. AP Lab Skills Guide

  3. AP Lab Skills Guide + Mix contents Begin Timing Tube 1 (substrate) Tube 2 (enzyme) Tube 3 (tubes 1 + 2) Begin Timing H2O2 Guaiacol Distilled H2O Peroxidase Distilled water

  4. AP Lab Skills Guide Each minute for 5 min compare the color of tube 3 to the standard Tube 3 (tubes 1 + 2) Begin Timing Graph the data…

  5. AP Lab Skills Guide Each minute for 5 min compare the color of tube 3 to the standard You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….

  6. AP Lab Skills Guide

  7. AP Lab Skills Guide Tube 5 Tube 6 Tube 1 Tube 2 Tube 3 Tube 4 Peroxidase H2O2 pH 12 buffer Peroxidase H2O2 pH 10 buffer Peroxidase H2O2 pH 8 buffer Peroxidase H2O2 pH 4 buffer Peroxidase H2O2 pH 6 buffer Peroxidase H2O2 pH 2 buffer Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...

  8. AP Lab Skills Guide Of course, we should combine the class data, take averages, SD, SE and graph with error bars…

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