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TEM

TEM. FF. Electron Microscopy Unit. Electron Microscopy Unit. SEM. Electron Microscopy Unit. Annual Report 2001/2002. IA. ENTER. This annual report (March 2001 to February 2002) of the Electron Microscopy (EM) Unit would take a pictorial format.

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TEM

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  1. TEM FF Electron Microscopy Unit Electron Microscopy Unit SEM Electron Microscopy Unit Annual Report 2001/2002 IA ENTER

  2. This annual report (March 2001 to February 2002) of the Electron Microscopy (EM) Unit would take a pictorial format. Relax and browse through our Diary of Events, accompanied by the music! Prof Mary Ng Head, EMU Compliments from the team of the EM Unit Micky Pat Suat Hoon “Da Jie” Deborah Boon Harn “Xiao Mei”

  3. Closing of Accounts

  4. 19th April Biomedical Science Workshop for teachers

  5. 18th May Image Pro Express Seminar and Hands-on Session (Organised with Media Cybernetics and Micro Lambda)

  6. The TCS-SP2 Confocal Microscope 21st May MOU between the EM Unit and Leica Microsystems Launch of TCS-SP2 Confocal Microscope

  7. 22nd to 24th May Training of users

  8. 11th to 15th June Image Analysis Course (With Carl Zeiss Singapore and Australian Key Centre for Microscopy and Microanalysis, University of Sydney, Australia)

  9. Installation of JEM 1010 With Gatan Digital camera (Dual View)

  10. 24th to 25th August Biotech Fair at Singapore Science Centre

  11. IMAGES from ELECTRON MICROSCOPY UNIT FACULTY OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE Artistic micrograph CD (available on request)

  12. 24th September Installation of XGT-2700 X-Ray Analytical Microscope (Horiba Instrument) Installed this microscope for 3 months for users to evaluate

  13. 25th to 27th September Imaging School with Gatan Training on how to use the digital camera attached to TEM Participants were from Taiwan, Korea and ASEAN region

  14. 27th September Visit by Mr Horiba, President of Horiba Instruments Dean came by to say hi

  15. 10th October Preparation for the 2nd NUS-JEOL Electron Microscopy Seminar

  16. 5th to 6th November 2nd NUS-JEOL Electron Microscopy Seminar and Workshop Micrograph Competition

  17. Barry Pereira (Department of Orthopedic Surgery, NUS) 2nd Most Artistic SEM (Life Science) Capillary loops of human index digit Waltzing Couples Lau Boon Harn (Electron Microscopy Unit, NUS) Best SEM Content (Life Science) Pollen of Barbados Nut Spiked Tyre Micky Leong (Electron Microscopy Unit, NUS) 3rd Most Artistic SEM (Life Science) Pollen of Hibiscus

  18. Justin Chu (Department of Microbiology, NUS) Best TEM Content (Life Science) West Nile (Sarafend) virus Mary Ng Mah Lee (Department of Microbiology, NUS) Most Artistic TEM (Life Science) FF of Immortalized Mouse Dendritic Cell Shoe print on the sand Squirrel Tan Suat Hoon (Electron Microscopy Unit, NUS) 2nd Most Artistic TEM (Life Science) Pineal gland of rat Wang Xin Sheng (Department of Anatomy, NUS) 2nd Best TEM Content (Life Science) Monkey Brain

  19. Renovations to the Air-conditioning System of the Unit The Unit had to close for the most of the month All equipment were shut-down and covered to protect them from the dust and debris that littered the place

  20. 11th December Visit from Mr Takashi Takeguehi, Chairman of JEOL Ltd

  21. 21st to 25th January Installation of DITABIS Imaging Plate System from Hi-Tech Instruments for one week of evaluation by users

  22. Summary of Achievements Publications Visitors Earnings New Equipment purchased with EM Unit’s earnings SKIP

  23. Publications • UROPS project • Light and scanning electron microscopic evaluation of Glyde File Prep in smear • layer removal - T.S. Lim, T.Y. Wee, M.Y. Choi, W.C. Koh & V. Sae-Lim • (in preparation for publication) • Honours dissertation • Cruising of West Nile (Sarafend) virus along microtubule highway • - Justin Chu Jang Hann • The effects of intermedilsin on neutrophils • - Kenneth Wong Hok Sum • A study on the effect of lactobacillus on murine bladder tumour growth • - Lim Boon Kian

  24. MSc thesis • Monitoring and Control of Biofilm growth using flow cell systems and molecular techniques - Pang Chee Meng • Cytotoxic effects of Garcinia gaudichaudii on cancer cells in culture and tumours in mice - Wu Xiao Hua • Microbial study of extended shelf life system for food – Lau Thye Ngak Matthew • Ph D thesis • An in vitro study of human immune response and neutralising mechanism for dengue virus infection - Se Thoe Su Yun • Biological markers of cell proliferation and apoptosis in nasopahryngeal cancer - Anita Jayasurya • Metallothionein expression in invasive ductal breast carcinoma - Jin Rongxian

  25. International refereed publication • Ananth C, Thameem Dheen S, Gopalakrishnakone P, Kaur C. Domoic acid • induced neuronal damage in the rat hippocampus: changes in apoptosis related • genes (bcl-2, bax, caspase-3) and microglial response. • J Neurosci Res. 2001 Oct 15;66(2):177-190. • Aiping Fang, HouTee Ng, Sam Fong Yau Li. Anchoring of Self-Assembled • Hemoglobin Molecules on Bare Indium-Tin Oxide Surfaces. • Langmuir, 14, 4360-4366. • ML Ng, SH Tan, JJH Chu. Transport and budding at two distinct sites of visible • nucleocapsids of West Nile (Sarafend) virus. • J. Medical Virology 2001; 65: 758-764. • Se Thoe SY, AE Ling, MML Ng. Alternative of virus entry mode a • neutralization mechanism for dengue-2 virus. • Journal of Medical Virology 2000; 62(3): 364-376. • Kumar SC, MML Ng. Antitumour activity of oral administration of mycovirus • extract from Lentinus edodes (Berk) Sing. (Agaricimycetideae) on murine • lymphoma. • International Journal of Medicinal Mushrooms 2000; 2(2): 125-132.

  26. Zaman V, JLC Howe, MML Ng. Scanning electron microscopy of Chilomastix • mesnili: (Wenyon, 1910) A lexieieff, 1912. • Parasitology Research 2000; 86: 327-329. • Yap AT, MML Ng. An improved method for the isolation of lentinan from edible • and medicinal shiitake mushroom, Lentinus edodes (berk) Sing. • (Agarico mycetideae). • International Journal of Medicinal Mushrooms 2001; 3: 9-19. • Lok SM, MML Ng, J Aaskov. Amino acid and phenotypic changes in dengue-2 • virus associated with escape from neutralization by IgM antibody. • Journal of Medical Virology 2001; 65: 315-323 (accepted for publication) • Tham M, Sim MK, Tang FR. Location of renin-angiotensin system components • in the hypoglossal nucleus of the rat. • Regulatory Peptides 2001; 101:51-57. • Jin R, Bay BH, Chow VTK. Metallothionein 1F mRNA expression correlates with • histological grade in breast carcinoma. • Breast Cancer Res Treat. 2001 Apr; 66(3):265-272. • Lim BK, Mahendran R, Lee YK, Bay BH. Chemopreventive effect of lactobacillus • rhamnosus on growth of a subcutaneously implanted bladder cancer cell line in • the mouse. Jap. J. Cancer 2002; Res.; 93.

  27. JT Schantz, DW Hutmacher, KW Ng, HL Khor, TC Lim, SH Teoh. Evaluation • of a Tissue Engineered Membrane-Cell Construct for Guided Bone Regeneration. • Accepted for Publication in International Journal of Oral and Maxillofacial • Implants JOMI • Swamy S Muthu & Tan BKH. Extraction, isolation, characterisation of active • antitumour principle, alpha-hederin, in Nigella sativa seeds. • Planta Medica. 2001; 67:29-32. • M.C. Phoon, Charles Desbordes, J. Howe, Vincent T.K. Chow. Linoleic and • Linolelaidic Acids differentially influence proliferation and apoptosis of Molt-4 • Leukaemia Cells. • Cell Biology International. 2001; 25(8):777-784. • JA van Kan, AA Bettiol, BS Wee, TC Sum, SM Tang and F Watt. Proton Beam • Micromachining: A new tool for precision 3D microstructures. • Sensors and Actuators A. 2001; 92:370-374. • JA van Kan, AA Bettiol and F Watt. High precision 3D metallic microstructures • produced using Proton Beam Micromachining. • Nuclear Instruments and Methods in Physical Research B. 2001; 181:258-262. • E.J. Teo, T. Osipowicz, A.A. Bettiol, J. van Kan, F. Watt, M.S. Hao, S.J. Chua. • Channeling Contrast Microscopy on Lateral Epitaxial Overgrown GaN film. • Nuclear Instruments & Methods in Physics Research Section B. 2001; 181: • 231-237.

  28. A.A. Bettiol, J.A. van Kan, T.C. Sum and F. Watt. A LabVIEW Based Scanning • and Control System for Proton Beam Micromachining. • Nuclear Instruments & Methods in Physics Research Section B. 2001; 181:49-53. • JA van Kan, AA Bettiol K. Ansari, F. Watt. Micromachining using a focused • MeV proton beam for the production of high precision 3D microstructures with • vertical side-walls of high orthogonality. • Proceedings of SPIE Volume. 2001; 4343:466-472. • A.M.A. Nasirudeen, Kevin SW Tan, M Singh and EH Yap. Programmed cell • death in a human intestinal parasite, Blastocystis hominis. • Parasitology. 2001; 123:235-246. • Ong WY, Kumar U, Switzer RC, Sidhu A, Suresh G, Hu CY, Patel SC. • Neurodegeneration in Niemann-Pick type C disease mice. Exp Brain Res. • 2001 Nov; 141(2):218-231. • Hu CY, Ong WY, Sundaram RK, Chan C, Patel SC. Immunocytochemical • localization of apolipoprotein D in oligodendrocyte precursor-like cells, • perivascular cells, and pericytes in the human cerebral cortex. J Neurocytol. • 2001 Mar; 30(3):209-218. • Lu XR, Ong WY, Halliwell B. The phospholipase A2 inhibitor quinacrine • prevents increased immunoreactivity to cytoplasmic phospholipase A2 (cPLA2) • and hydroxynonenal (HNE) in neurons of the lateral septum following fimbria- • fornix transection. Exp Brain Res. 2001 Jun; 138(4):500-508.

  29. Lu XR, Ong WY. Heme oxgenase-1 is expressed in viable astrocytes and • microglia but in degenerating pyramidal neurons in the kainate-lesioned rat • hippocampus. Exp Brain Res. 2001 Apr; 137(3-4):424-431. • Ng CH, Ong WY. Increased expression of gamma-aminobutyric acid transporters • GAT-1 and GAT-3 in the spinal trigeminal nucleus after facial carrageenan • injections. Pain. 2001 May; 92(1-2):29-40. • Cao Q, Ong WY, Halliwell B. Lipid peroxidation in the postnatal rat brain. • Formation of 4-hydroxynonenal in the supraventricular corpus callosum of • postnatal rats. Exp Brain Res. 2001 Mar; 137(2):205-13.

  30. Conference papers • Yap AT, MML Ng. Inhibition of human colon carcinoma by lentinan from • Shiitake mushroom (Lentinus edodes) (abstract). • Presented at First International Conference on Asian Therapies for Cancer, • 1-3 March 2001, The Roosevelt Hotel, United States. • Chua JJE, VTK Chow, MML Ng. Construction of novel candidate DNA vaccines • against dengue virus (abstract). • Scientific Program/Abstracts of the Sixth International Symposium on Positive • Strand RNA Viruses compiled by M. Brahic et al; pp2-126. • Presented at Sixth International Symposium on Positive Strand RNA Viruses, • 28 May-2 June 2001, Institut Pasteur, Paris, France. • Chu JJH, MML Ng. Transportation of envelope and capsid proteins of flavivirus • West Nile (Sarafend) along microtubules highway (abstract). • Scientific Program/Abstracts of the Sixth International Symposium on Positive • Strand RNA Viruses compiled by M. Brahic et al; pp1-90. • Presented at Sixth International Symposium on Positive Strand RNA Viruses, • 28 May-2 June 2001, Institut Pasteur, Paris, France.

  31. Chua JJE, VTK Chow, MML Ng. Construction of novel candidate DNA vaccines • against dengue virus (abstract). Academia as the foundation for the Biomedical • Sciences compiled by SG Lim; pp88. • Presented at Fifth NUH-NUS Faculty of Medicine Annual Scientific Meeting, • 29-30 June 2001, Clinical Research Centre, Singapore. • MML Ng, JT Schantz, P Netto, DW Hutmacher. Evaluation mineralisation in • tissue engineered bone samples by using X-ray fluorescence spectrometry • (abstract). pp84. • Presented at International Conference on Materials for Advanced Technologies, • 1-6 July 2001, Singapore International Convention and Exhibition Centre, • Singapore. • Chua JJE, VTK Chow, MML Ng. Stability of cloned dengue virus genes in • recombinant E. coli host (abstract). Presented at Biomedical Asia 2001, 19-21 • September 2001, Singapore International Convention and Exhibition Centre, • Singapore. • Yap AT, MML Ng. The role of lentinan in the inhibition of cancer development • (abstract). Presented at Biomedical Asia 2001, 19-21 September 2001, Singapore • International Convention and Exhibition Centre, Singapore. • Chu JJH, MML Ng. Egression of flavivirus West Nile (Sarafend) from polarized • epithelial cells is restricted to the apical surface (abstract). • Presented at Biomedical Asia 2001, 19-21 September 2001, Singapore • International Convention and Exhibition Centre, Singapore.

  32. MML Ng, JJH Chua. Dynamic role of microtubules in West Nile virus replication. • Presented at Advances in the application of electron microscopy for medical • diagnostic, 8-10 November 2001, Palace of the Golden Horses, MINES, • Kuala Lumpur, Malaysia. • ML Ng, AGL Koh, JJE Chua. The use of antigen-presenting cells to study the • possible immunological profile of dengue virus infection. Presented at 3rd ASEAN • Microscopy Conference, 30 January-1 February 2002, Chiang Mai, Thailand. • JJH Chu, ML Ng. West Nile virus a hitchhiker of host cell cytoskeleton. • Presented at 3rd ASEAN Microscopy Conference, 30 January-1 February 2002, • Chiang Mai, Thailand. • Bay BH. Biological markers of nasopharyngeal cancer. • Presented at 10th EMSM Malaysia, 8-10 November 2000, Kuala Lumpur, • Malaysia. • Hsu Chin-Ying, Stephen, Veerappan Girija. A Novel Technique for • Characterization Of Lipid in Mature Enamel Using Laser Scanning Confocal • Microscopy. Presented at Focus on Microscopy 2002, 7 April 2002, Kaohsiung, • Taiwan. • Chew Joon Lin, Huang Fu T.Q., Mao Hai Quan, Kam W. Leong, Chua Kaw Yan. • Oral delivery of house dust mite allergen gene using chitosan-DNA nanoparticles. • Presented at 3rd Malaysian Congress of Allergy and Immunology, 25-27 Jan 2002, • Kuala Lumpur, Malaysia.

  33. JT Schantz, M Endres, TC Lim, SH Teoh, J Chong, M Ng, DW Hutmacher. • Repair of defects with customized tissue-engineered bone grafts. • Presented at 11th Asean Congress of Plastic Surgery, 30 January-2 February 2002, • Singapore • M.C. Phoon, Charles Desbordes, J. Howe, Vincent T.K. Chow. Linoleic and • Linolelaidic Acids affect the proliferation and ultrastructure of MOLT-4 T- • Lymphoblastic Leukaemia Cells (abstract). • Book of Abstracts on 2nd Combined Annual Scientific meeting, “New Therapies • and Challenges in Biomedical Sciences”; pp54. • Presented at 2nd Combined Annual Scientific meeting, “New Therapies and • Challenges in Biomedical Sciences”, 8-9 September 2000, Singapore. Back

  34. Visitors Yuri Bobryshen St. Vincent’s Hospital University of New South Wales Australia Michael Philpott Material Science National University of Singapore Ping-chin Cheng State University of New York Buffalo, NY Sharlene Anthony Science Centre Singapore Henry Lim Horiba Instruments Han Hock Chuan Horiba Instruments Athena Teng Horiba Instruments Ahmad Fauzi M. N. University Sains Malaysia Perak Campus Buhari A. Oyofu Namru-2 Jakarta, Indonesia

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