Molecular Characterization of the Canine HMGB1. Presented by Melissa Metcalfe and Shauna Maguire. Canine Genome. An overview. First Sequenced by Celera with the DNA from Shadow, a male Standard Poodle, in 2002. (WGS 80% coverage rough draft)
Presented by Melissa Metcalfe and Shauna Maguire
Next major sequencing, done by the Broad Institute, was of Tasha, a 7 year old female boxer. (95% coverage)
Performed to define a basic expression pattern of the protein in canine
heart, lung, muscle, kidney, and spleen tissue.
Lane 1 – Kidney
Lane 2 – Spleen
Lane 3 – Spleen
Lane 4 – Heart
Lane 5 – Heart
Lane 6 – lung
Lane 7 – Muscle
Lane 8 - Fibroblasts
Makes it possible to map any cloned locus to its position on the metaphase chromosome.
Good for quickly obtaining the chromosomal position of a newly cloned locus. Does not require mapping to be “in relation” to another.