Multiplex autoantibody profiling for autoimmune diseases and cancer. April, 2, 2014. Profile antibodies to over 10,000 human proteins using Luminex xMAP™ technology. Jim Lazar, Ph.D. VP, Assay Development. OriGene Introduction. OTI Locations Rockville (HQTs/R&D) Seattle (Blue Heron)
Multiplex autoantibody profiling for autoimmune diseases and cancer
April, 2, 2014
Profile antibodies to over 10,000 human proteins using Luminex xMAP™ technology
Jim Lazar, Ph.D.
VP, Assay Development
What is EarlyCDT–Lung?
EarlyCDT-Lung is a simple, physician-ordered, diagnostic blood test that measures the presence of autoantibodies to a panel of seven lung cancer-associated antigens.
Identification of many targets from a small number of samples
& Quantitative Evaluation
Large sample #s
Refine target list
Any of OriGene’s >10,000 purified proteins can be custom-coupled to Luminex beads
Protein-coupled Luminex Beads
Sample containing human IgG
Mix with Anti-human PE Conjugate
Read Signal in Luminex
Multiple profiling arrays can be mixed together and analyzed as a larger multiplex
Mix with beads, incubate
Total time – about 2.5 hours for 96 samples
Up to 100 results per well
Wash, add conj. & incubate
Read in Luminex
Autoantibodies to tailor-made panels of tumor-associated antigens in breast carcinoma.E Piura and B PiuraJ Oncol, Jan 2011; 2011: 982425.
Seromic profiling of ovarian and pancreatic cancer.
S. Gnjatic S, et al.
PNAS U S A. Mar 2010 16;107(11):5088-93.
A Distinct Repertoire of Autoantibodies in Hepatocellular Carcinoma Identified by Proteomic Analysis
François Le Naour, et al.
Mol. Cell. Proteomics, Mar 2002; 1: 197 - 203.
Multiple serological biomarkers for colorectal cancer detection.
CC Chan, et al.
Int J Cancer, Apr 2010; 126(7): 1683-90.