shotgun metagenomic sequencing

1. Shotgun Metagenomic Sequencing Microorganisms are present everywhere in nature. Studies reveal that microbes are critical to these environments and play an essential part in the ecosystem. It arouses an attention for the interpretation of taxonomic composition of a complex microbial community. Shotgun sequencing has been proven as a fast sequencing strategy that uses random fragments. Metagenomic shotgun sequencing is a rapid and powerful tool for obtaining all genetic information in all organisms within a microbial community. Shotgun metagenomic sequencing targets the entirety of the microbial genetic information contained in an environmental sample. The obtained community taxonomic profile can be further associated with the functional profile of known and unknown organism lineages. The complete sequences of protein- coding genes and full operons in the sequenced genomes can offer invaluable functional knowledge about the microbial communities inhabiting practical ecosystems under study. Metagenomic shotgun sequencing may provide genetic information on potentially novel biocatalysts or enzymes, genomic linkages between function and phylogeny for uncultured organisms, evolutionary profiles of community function and composition and much more. 16S/18S/ITS Amplicon Sequencing is another alternative for metagenomics studies. But this method only interprets the microbial biodiversity, without insights into function. Learn more about genotyping by sequencing at: https://www.cd-genomics.com/Metagenomic-Shotgun-Sequencing.html