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Figure S6 – page 1. 50%>X>20%. X<20%. X>50%. Activity (UDP-Gal). A. B. C.

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Figure S6 – page 1

50%>X>20%

X<20%

X>50%

Activity

(UDP-Gal)

A

B

C

Figure S6Aligned structures of altered residues that show no apparent affect for both UDP-Glc and UDP-GlcNAc bound structures. (A) p.A25V. (B) p.P293L. (C) p.L313M.Original residues are coloured deep teal while altered residues are coloured pink. Highlighted residues, cofactors and substrates are depicted as stick figures. WT and mutant structures are depicted as cartoon figures and are coloured wheat and light blue respectively. Oxygen, nitrogen and phosphate atoms are coloured red, blue and orange respectively. Figures were created using PyMol (www.pymol.com) and the appropriate energy minimised structures of 1EK5 and 1HZJ.


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