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Chem/Biol 473 S. Schultze and G. Prody

Chem/Biol 473 S. Schultze and G. Prody Figure 5-21 The central dogma of molecular biology. Page 93 How do RNA and DNA differ? Figure 5-6 Bacteriophages attached to the surface of a bacterium. Page 84 Figure 5-7 Diagram of T2 bacteriophage injecting its DNA into an E. coli cell.

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Chem/Biol 473 S. Schultze and G. Prody

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  1. Chem/Biol 473 S. Schultze and G. Prody

  2. Figure 5-21 The central dogma of molecular biology. Page 93

  3. How do RNA and DNA differ?

  4. Figure 5-6 Bacteriophages attached to the surface of a bacterium. Page 84

  5. Figure 5-7 Diagram of T2 bacteriophage injecting its DNA into an E. coli cell. Page 84

  6. Figure 5-8 The Hershey-Chase experiment. Page 85

  7. You need to be able to draw and name • their respective nucleosides and • the nitrogen containing bases (what are they) • their respective nucleotide mono-, di- and triphosphates You also need to remember amino acid structures, HH, MM kinetics, thermodynamics, etc. from 471,2.

  8. Figure 5-1 Chemical structures of (a) ribonucleotides and (b) deoxyribonucleotides. Page 81

  9. Table 5-1 Names and Abbreviations of Nucleic Acid Bases, Nucleosides, and Nucleotides. Page 86

  10. Figure 5-2 Chemical structure of a nucleic acid. Page 82

  11. Figure 5-19 Electron micrograph of a T2 bacteriophage and its DNA. Page 91

  12. Figure 29-17 Electron micrographs of circular duplex DNAs. Their conformations vary from no supercoiling (left) to tightly supercoiled (right). Page 1122

  13. Figure 29-24 Agarose gel electrophoresis pattern of SV40 DNA. Page 1126

  14. Table 5-2 Sizes of Some DNA Molecules.

  15. Figure 5-14 Schematic representation of the strand separation in duplex DNA resulting from its heat denaturation. Page 90

  16. Figure 5-15 UV absorbance spectra of native and heat-denatured E. coli DNA. Page 90

  17. Figure 5-16 Example of a DNA melting curve. Page 90

  18. Figure 5-17 Variation of the melting temperatures, Tm, of various DNAs with their G + C content. Page 91

  19. Figure 5-10 X-ray diffraction photograph of a vertically oriented Na+ DNA fiber in the B conformation taken by Rosalind Franklin. Page 87

  20. Figure 5-11 Three-dimensional structure of B-DNA. Page 87

  21. Figure 5-12 Watson-Crick base pairs. Page 88

  22. Figure 29-1a Structure of B-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis. Page 1108

  23. Figure 29-1b Structure of B-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis. Page 1109

  24. Figure 29-2a Structure of A-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis. Page 1110

  25. Figure 29-2b Structure of A-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis. Page 1111

  26. Figure 29-3a Structure of Z-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis. Page 1112

  27. Figure 29-3b Structure of Z-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis. Page 1113

  28. DNA tutorial link http://molvis.sdsc.edu/dna/index.htm

  29. Figure 29-7 The conformation of a nucleotide unit is determined by the seven indicated torsion angles. Page 1116

  30. Figure 29-8 The sterically allowed orientations of purine and pyrimidine bases with respect to their attached ribose units.

  31. Figure 29-10b Nucleotide sugar conformations. (b) The C2-endo conformation, which occurs in B-DNA. Page 1117

  32. Table 29-1 Structural Features of Ideal A-, B-, and Z-DNA.

  33. Figure 5-13 Demonstration of the semiconservative nature of DNA replication in E. coli by density gradient ultracentrifugation. Page 89

  34. Figure 5-31 Action of DNA polymerases. Page 99

  35. Figure 5-32a Replication of duplex DNA in E. coli. Page 100

  36. Figure 5-32b Replication of duplex DNA in E. coli. Page 100

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