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T0 5 h. T0 5 h. T0 5 h. M. T0 5 h M. D. 1 2. 1 2. 1 2. 1 2. A. 95. 36. 72. 28. NaCl. 55. 300 mM. 50 mM. 75 mM. 150 mM. Ros-gami. HMS Ros2. BL21 Ros2. HMS LysE. HMS Rossetta 2. 95. pH. 72. T0 5 h - 5 h + M.
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T0 5 h T0 5 h T0 5 h M T0 5 h M D 1 2 1 2 1 2 1 2 A 95 36 72 28 NaCl 55 300 mM 50 mM 75 mM 150 mM Ros-gami HMS Ros2 BL21 Ros2 HMS LysE HMS Rossetta 2 95 pH 72 T0 5 h- 5 h+M T0 5 h- 5 h+ T0 5 h- 5 h+ B 55 pH 6.0 pH 7.0 pH 8.0 pH 9.0 72 E 55 Ros-gami BL21 Ros2 HMS Ros 2 37 ºC 21 ºC M T0 T0 5 h- 5 h+M T0 5 h- 5 h+ T0 5 h- 5 h+ S P S P C 72 72 55 55 HMS Rossetta 2 RP 2 HMS Lys E HMS 174
![Theoretical [M+H]+ ion m/z](https://cdn1.slideserve.com/3188132/slide1-dt.jpg)
