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激光诱导荧光检测器 Laser Induced Fluorescence Detector

激光诱导荧光检测器 Laser Induced Fluorescence Detector. 市场部. TriSep TM -2100 激光诱导荧光检测器. 原理图. 转向反射镜. 计算机. 带通滤光片. 激发光滤光片. 分色滤光片. 激光器. 显微物镜. 毛细管. 特点. 最新激光技术 高能量高稳定性的固体激光器微激发光源 独特光路设计 低噪声与基线漂移 高灵敏度、宽动态范围( 单光子技术 PMT ) 1ⅹ10 -12 M 根据用户需求更换光源及波长 保证最大的荧光强度. 技术参数. 软件界面. 应用实例.

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激光诱导荧光检测器 Laser Induced Fluorescence Detector

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  1. 激光诱导荧光检测器Laser Induced Fluorescence Detector 市场部

  2. TriSepTM-2100 激光诱导荧光检测器

  3. 原理图 转向反射镜 计算机 带通滤光片 激发光滤光片 分色滤光片 激光器 显微物镜 毛细管

  4. 特点 • 最新激光技术 高能量高稳定性的固体激光器微激发光源 • 独特光路设计 低噪声与基线漂移 • 高灵敏度、宽动态范围(单光子技术PMT) 1ⅹ10-12M • 根据用户需求更换光源及波长 保证最大的荧光强度

  5. 技术参数

  6. 软件界面

  7. 应用实例 • 毛细管电色谱仪 • 毛细管电泳仪 • 微径液相色谱仪 • 高效液相色谱 超强匹配性

  8. HPLC-LIF 检测FITC柱前衍生脂肪胺 色谱柱: Unimicro Kromasil C18 5μm 250×4.6mm 流动相: A) 40mM Na2HPO4:H2O: 1% H3PO4=10:90:0.1 B) 40mM Na2HPO4:H2O: ACN:1% H3PO4=10:25:65:0.1 梯度条件: 0~25min A相从70%降到 0% 流速: 1.0ml/min 激发波长: 473nm 发射波长:520nm

  9. 毛细管电泳- LIF 检测 FITC 衍生的氨基酸 毛细管:100μm i.d.×58cm 缓冲液: 10mM borate buffer (pH9.3) 分离电压:10kV 激发波长:473 nm 发射波长:520 nm 进 样: 10kV,2S 样 品: 7×10-9M AA

  10. 加压毛细管电色谱-LIF 检测FITC 衍生的14种脂肪胺类化合物 毛细管柱: EP100-20-45-3 C18 流动相: (A)10%(v/v) ACN + 10% 40mM NaH2PO4 +100ul 1%H3PO4+H2O (B)65%(v/v) ACN + 10% 40mM NaH2PO4 +100ul 1% H3PO4+ H2O 梯度条件: 0~30min 100% to 0% A 压 力: 10 MPa 分离电压: 5 kV 激发波长:473 nm, 发射波长:520 nm 样 品: Aliphatic Amines C1~C14

  11. Thank You!

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