Investigation of the use of siderophores from pseudomonas genus to chelate heavy metal ions
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Investigation of the Use of Siderophores from Pseudomonas genus to chelate Heavy Metal ions. Members: Vamsi Meka Josh Hasan Shaun Png Johnny Yeung. 6/11/2009. Problem.

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Investigation of the use of siderophores from pseudomonas genus to chelate heavy metal ions

Investigation of the Use of Siderophores from Pseudomonas genus to chelate Heavy Metal ions



Josh Hasan

Shaun Png

Johnny Yeung



  • Arsenic poising in the water of the village of Bangladesh and formation of lung cancer in patients causes health problems (Renshawetal. 2002).

  • Heavy metal ions are not biodegradable nor thermodegradable and are toxic. (Tansupo et al. , 2009)

  • Metal pollution caused by factors such as motor vehicle emissions, sewage sludge applications, and manufacturing has led to formation of hazardous environments

Background siderophores
Background - siderophores

  • The property of siderophores in chelating ferric ions have long been known and utilized in scientific industries (Duckworth & Sposito, 2007)

  • Contains antimicrobial properties (Barry & Challis, 2009).


  • Siderophores will demonstrate chelation for nickel, copper(II), iron(III) and lead(II) ions.

  • Temperature and pH for the greatest degree of chelation is 26 degrees Celcius and 7.2 respectively.


  • The objective of the experiment is

    • To investigate the degree of chelation of heavy metal ions of copper (II), iron (III), nickel and lead(II) by siderophores from Pseudomonas genus

    • To find the temperature and pH which allows the greatest degree of chelation.


Part I: Investigating which heavy metal ions can be chelated:

Phase 1: Culturing the bacteria


Phase 2: introducing siderophores to solution.

Step 4: Prepare 3 solutions as follows:

Set up 1: Experimental Set up; Set up 2 and 3: controls

Set up 2: Determine if concentration of heavy metal ions changes in absence of Pseudomonas bacteria

Set up 3: Determine if heavy metal ions affect growth of Pseudomonas bacteria

In the experiment, only record the results for set ups 1 and 2 to compare the difference in heavy metal ion concentration.


For set ups 1 and 2,


Part II: Determining optimum temperature for chelation


Part III: Determining optimum pH for chelation

  • Repeat the same procedures for Part I for 5 sets for each type of bacteria, but during step 3, alter the pH to 5.2, 6.2, 7.2, 8.2, 9.2 for each set.

  • At the end of step 10, plot a graph of difference in concentration/ppmvs pH to determine the optimum pH for chelation.

Data collection
Data collection

  • Change in concentration/ppm

    • The higher the change, the higher the rate of chelation

  • Statistical tests

    • Done before and after introduction of siderophores from Psudomonasbacteria

    • p<0.05 suggests that the difference is insignificant and vice versa.

    • Sample size=5

    • Paired t-test to be done

Work distribution and safety issues
Work distribution and safety issues

  • Both AOS and HCI will take exactly the same steps (same heavy metal ion, same treatment etc.)

  • However, AOS would work with Pseudomonas syringae while HCI to work with Pseudomonas fluorescens. Both are at BSL level 1 so they are safe to work with.

  • Lab coats and gloves are worn at all times during experiments.


  • Collected Articles to use in Background Research

  • Background Research draft completed and peer reviewed by three people

  • Singaporean team has arrived in AOS and worked out details for project.

  • Both sides to work on their respective tasks while in AOS and HCI respectively and combine results and explanations when AOS arrives in HCI in August 2010.

  • A parallel project to ease combination in the future.


  • Barry, S.M., & Challis G.L. (2009). Recent advances in siderophore biosynthesis. Current Opinions in Chemical Biology, 13(2), 205-215

  • Cook, R. J. 1993. Making greater use of introduced microorganisms for biological control of plant pathogens. Annu. Rev. Phytopathol. 31:53–80.

  • Duckworth, O.W., & Sposito G. (2007). Siderophore-promoted dissolution of synthetic and biogenic layer-type Mn oxides. Chemical Geology, 242(3-4), 497-508

  • Miethke, M., & Marahiel M.A. (2007). Siderophore-based iron acquisition and pathogen control. Microbiology and molecular biology reviews, 71(3), 413-451

  • Nielands, J. B. (1995). Siderophores: structure and function of microbial iron transport compounds. The Journal of Biological Chemistry, 270(45), 26723-26726.

  • Renshaw, J.C., Robson, G.D., Trinci, A.P., Wiebe, M.G., & Livens, F.R. (2002). Fungal siderophores: structures, functions and applications. Mycological Research, 106(10), 1123-1142

  • Visca, P., Imperi, F., & Lamont, I.L. (2006). Pyoverdinesiderophores: from biogenesis to biosignificance. TRENDS in Microbiology, 15(11), 22-30