Investigation of the Use of Siderophores from Pseudomonas genus to chelate Heavy Metal ions. Members: Vamsi Meka Josh Hasan Shaun Png Johnny Yeung. 6/11/2009. Problem.
Part I: Investigating which heavy metal ions can be chelated:
Phase 1: Culturing the bacteria
Phase 2: introducing siderophores to solution.
Step 4: Prepare 3 solutions as follows:
Set up 1: Experimental Set up; Set up 2 and 3: controls
Set up 2: Determine if concentration of heavy metal ions changes in absence of Pseudomonas bacteria
Set up 3: Determine if heavy metal ions affect growth of Pseudomonas bacteria
In the experiment, only record the results for set ups 1 and 2 to compare the difference in heavy metal ion concentration.
For set ups 1 and 2,
Part II: Determining optimum temperature for chelation
Part III: Determining optimum pH for chelation