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a. b. c. d. e. f. h. g. i. j.

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  1. a b c d e f h g i j Supplementary 1: Aleurone isolation. a-b) Cutting the two ends of the seed. c) The middle cylindrical portion. d) Incubation of middle cylindrical portion overnight in sterile water. e) Opening the cylindrical portion from the groove. f) The cylindrical portion opens into a flat layer. g-h) Scrapping off the starchy endosperm. i) Isolated aleurone layer attached to seed coat. j) The aleurone layer after washing in sterile water.

  2. a b Supplementary 2: Amylase activity in a Rht3 carrier T. aestivum MBW400 a) Amylase activity in MBW400. b) Total protein secreted into the buffer in MBW400. Although total secreted protein levels are comparable with the three T. dicoccum varieties studied the amylase activity was negligible showing GA insensitivity.

  3. a b c Supplementary 3: Schematic diagram showing possible events before transcription of GA-responsive genes. a) In the tall genotype b) In the RhtB1bsemi-dwarf.

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