Cell structure and function
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Cell Structure and Function. Preview. Cell characteristics and cell theory Overview of cell membrane Eukaryotic cells Eukaryotic organelles Prokaryotic cells How Cells Move. Smallest unit of life Can survive on its own or has potential to do so Senses and responds to environment

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Cell Structure and Function

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Cell structure and function

Cell Structure and Function


Preview

Preview

  • Cell characteristics and cell theory

  • Overview of cell membrane

  • Eukaryotic cells

  • Eukaryotic organelles

  • Prokaryotic cells

  • How Cells Move


Characteristics of cells

Smallest unit of life

Can survive on its own or has potential to do so

Senses and responds to environment

Has potential to reproduce

Differ in:

Size

Shape

functions

Characteristics of Cells


Cell theory

Cell Theory

1) Every organism is composed of one or more cells

2) Cell is smallest unit having properties of life

3) Continuity of life arises from growth and division of single cells


Structure of cells

Structure of Cells

All cells have three distinct sections:

Plasma Membrane

Nucleus (or nucleoid region in prokaryotes)

Cytoplasm


Cell structure and function

Cell

6

  • Plasma Membrane

  • Nucleus

  • Cytoplasm


Preview of cell membranes

one layer

of lipids

one layer

of lipids

Preview of Cell Membranes

Plasma membranes are composed mostly of a lipid bilayer that prevents free passage of water soluble substances across it.


Plasma membrane

Plasma Membrane

8


Other names for a cell membrane

Other names for a cell membrane:

  • Cytoplasmic membrane

  • Semipermeable membrane

  • Phospholipid bilayer


Eukaryotic cells

Eukaryotic Cells

Have a nucleus 

Have organelles – small, membrane bound “organs” in the cell that perform a specific job for the cell.

Found in the Protista, Fungi, Plant, and Animal kingdoms

Have 1000 times more DNA than prokaryotic cells


Organelle functions

Organelle Functions

  • nucleus - controls the cell’s activities

    • Nuclear membrane – separates cytoplasm from nuclear material

    • Nucleolus – site of RNA and ribosome synthesis

    • DNA – genetic material

  • cytoplasm – Space between nucleus and plasma membrane

    • (cytosol) jellylike substance that fills the inside of a cell , gives structure and shape


Cell structure and function

Cont.

  • endoplasmic reticulum - "tunnels" in the cytoplasm that allow materials to move through the cell easier (subway system of the cell)

    • Rough (RER) – makes proteins, covered in ribosomes

    • Smooth (RER) - makes lipids, degrades fats, detoxifies material

  • ribosomes – attached to ER and scattered in cytoplasm, make proteins

  • mitochondrion – powerhouse, produce energy in the cell 

  • Golgi body - stores, processes, and secretes proteins and lipids (post office)


Cell structure and function

  • vesicle- sacs that transport material in cell

  • centrioles – centers that produce and organize structures that help in cell reproduction (animal cells only)

  • lysosome – digest, recycles nutrients (suicide sack)

  • cytoskeleton – structurally supports, gives shape & helps move cell components


Quick check

Quick Check

  • List two parts of the cell theory.

  • Rough endoplasmic reticulum is responsible for making ___ for the cell.

  • List the four eukaryotic kingdoms

  • Centrioles are only found in ___ cells.


Eukaryotic animal cell structure

Eukaryotic Animal Cell Structure


Plant vs animal cell

Plant vs. Animal Cell


Plant cells only

Plant Cells Only

  • cell wall - rigid surrounding of plant cells, protects, structural to support

  • chloroplasts - contain chlorophyll in plants; this is where the plant’s food is produced by photosynthesis

  • vacuoles - large bodies in plant cells that hold water, waste, etc.


A closer look at major organelles

A closer look at major organelles


A closer look at the nucleus

A closer look at the nucleus:

19


Nuclear envelope membrane

Nuclear Envelope/Membrane

20

  • Double lipid membrane with pores

  • Controls what goes in and out

    • Pores control ions & water soluble materials entrance and exit

  • Ribosomes on outer membrane

  • Membrane merges with ER

  • Nucleoplasm - semifluid interior of nucleus


Nuclear envelope

Nuclear Envelope


Nucleolus

Nucleolus

  • Mass of proteins that codes for rRNA (ribosomal RNA)

  • Synthesis of ribosomes and proteins


Genetic material dna

Genetic Material (DNA)

Chromosome – one DNA molecule and the many proteins that are associated with it

Chromatin – total collection of all DNA molecules and their associated proteins


Cell structure and function

  • DNA + proteins=

    chromatin

    Chromatin strands

    bunched together=

    chromosome


A closer look at er

A closer look at ER:

  • Endoplasmic reticulum

  • A flattened channel that starts at the nuclear envelope/membrane and folds back and forth

  • Two types

    Rough (RER)

    • Has ribosomes attached

      • Makes proteins

        Smooth (SER)

    • Detoxifies drugs, makes lipids


A closer look at golgi bodies

A closer look at Golgi bodies:

  • Vesicles pinch off of ER.

  • Fuse with Golgi bodies

  • Golgi bodies repackage and ship vesicles by adding or removing molecules to proteins and lipids.

  • Think post office

    and stacks of

    pancakes with

    syrup!


A closer look at lysosomes

A closer look at lysosomes:

  • membrane-enclosed vesicles that contain powerful digestive enzymes

    • internal pH reaches 5.0

  • Functions

    • digest foreign substances and recycles own organelles

    • Autolysis

    • Suicide sac


Tay sachs disorder

Tay-Sachs Disorder

Affects children of eastern European descent

Genetic disorder caused by absence of single lysosomal enzyme

enzyme normally breaks down glycolipid commonly found in nerve cells

as glycolipid accumulates, nerve cells lose functionality

chromosome testing now available


A closer look at mitochondria

A closer look at mitochondria:

  • Mitochondria resemble bacteria

    • Have DNA, ribosomes

    • Divide on their own

  • May have evolved from ancient bacteria that were engulfed but not digested

  • Mitochondrial DNA (genes) are usually inherited only from the mother.


Mitochondria cont

Mitochondria (cont.)

Double outer membrane

Inner folded membrane

Site of most of cells ATP production

Only in eukaryotic cells

Site of aerobic respiration (oxygen present)

Numerous in skeletonal cells


A closer look at chloroplasts and other plastids

A closer look at chloroplasts (and other plastids):

  • Plastids

  • Are organelles that function in photosynthesis or storage in plants.

  • Three types

    • Chloroplast

    • Chromoplasts

    • Amyloplasts


Cell structure and function

Cont.

33

Chloroplast –

  • Conduct photosynthesis

  • Enclosed by a double membrane

  • Thylakoid stacks of grana

    • Contain pigments such as chlorophyll

  • Stroma fluid filled area


Other plastids

Other Plastids

Chromoplasts

No chlorophyll

Abundance of carotenoids

Color fruits and flowers red to yellow

Amyloplasts

No pigments

Store starch (tubers- potatoes)


A closer look at plant cell walls

A closer look at plant cell walls:

  • Surrounds the plasma membrane

  • Protects, supports and give shape to cell

  • Porous – allows water and solutes to pass in/out

  • In all plants

  • Some protist and fungi

  • Cuticle on outer most surfaces of plants


Even cells have a skeleton

Even Cells Have a Skeleton

36

  • Cyto means cell

    • So cytoskeleton means cell skeleton

  • It is organized system of protein filaments in the cytoplasm.

  • Some are permanent others are temporary


Cytoskeletal elements

Cytoskeletal Elements

intermediate

filament

microtubule

microfilament


Functions of cytoskeletal elements

Functions ofCytoskeletal Elements:

  • Move organelles within the cytoplasm

  • Assist in cell division

  • Provide structure and support for the cell

  • Can be used to identify cells


Prokaryotic cells

Prokaryotic Cells

DNA is not enclosed in nucleus

Generally the smallest, simplest cells

No organelles

Most ancient form of life

Archaeans and bacteria the only representatives

Prokaryotic means “before the nucleus


Prokaryotic cells1

Prokaryotic Cells

  • Permeable, semi-rigid cell wall outside plasma membrane gives shape

  • Plasma membrane- semi-permeable to control what goes in and out

    • Can contain pigments for photosynthesis

  • Polysaccharides are on surface to help them stick to objects or give a protective coating

  • 1-2 Flagella – movement


Prokaryotic cells2

Prokaryotic Cells

  • Pili – helps stick to surfaces and exchange genetic material

  • Cytoplasm – semifluid material inside cell

  • Ribosomes – scattered in cytoplasm, protein making site

  • Nucleoid – concentrated region where DNA is located. DNA is circular.

  • Plasmids – scattered in cytoplasm, these can confer selective advantages such as antibiotic resistance. Contain just a few genes.


Prokaryotic structure

Prokaryotic Structure

pilus

cytoplasm

with ribosomes

DNA

flagellum

capsule

cell wall

plasma membrane


Quick check1

Quick Check


How do cells move

How Do Cells Move?

  • Cells must have ATP in order for movement to take place.

  • Cilia, flagella and false feet are all ways that cells move.


Cilia flagella and false feet

Cilia, Flagella and False Feet

  • Cilia

    • many small projections on cell membranes working in together for movement

    • Along trachea, oviducts in humans

  • Flagella

    • generally 1-2 projections that move an object

    • Sperm is the only flagella in humans

  • Pseudopodia

    • False foot

    • Temporary extension of cytoplasm for movement


Protists use all three

Protists use all three.

46

Ciliapseudopodia

flagella


Cell structure and function

Lab Notes


Microscopes

Lead to the ability to develop the Cell Theory

Create detailed images of something that is otherwise too small to see

Light microscopes

Simple or compound

Uses two sets of lenses to magnify the living or dead image

Electron microscopes

Transmission EM or Scanning EM

Uses electrons view either inside or surface of a dead cell

Microscopes

48


New terms

New Terms

Wavelength – distance from the peak of a wave to the peak of another wave

Ocular Lens enlarges 10x inside the eye piece

Objective lens magnify at various levels

Stage supports the object viewed on slide

49


Microscopes1

Create detailed images of something that is otherwise too small to see

Light microscopes

Simple or compound

Electron microscopes

Transmission EM or Scanning EM

Microscopes

50


Limitations of light microscopy

Limitations of Light Microscopy

Wavelengths of light are 400-750 nm

If a structure is less than one-half of a wavelength long, it will not be visible

Light microscopes can resolve objects down to about 200 nm in size

51


Electron microscopy

Electron Microscopy

Uses streams of accelerated electrons rather than light

Electrons are focused by magnets rather than glass lenses

Can resolve structures down to 0.5 nm

53


Sem surface views

SEM- surface views

54


Electron microscopes

Electron Microscopes

TEM

SEM

55


Tem inside cell view

TEM- inside cell view

56


Image comparison

Image comparison

The electron microscope allows a smaller object to seen

The electron microscope is not limited by the wavelength of light

57


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