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DTI Basics – Water Diffusion

DTI Basics – Water Diffusion. Einstein on Brownian Motion. 1905 five important papers. Why MRI : Detection of Acute Stroke. “Diffusion Weighted Imaging (DWI ) has proven to be the most effective means of detecting early strokes” Lehigh Magnetic Imaging Center. Conventional T 2 WI.

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DTI Basics – Water Diffusion

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  1. DTI Basics – Water Diffusion Einstein on Brownian Motion 1905 five important papers

  2. Why MRI : Detection of Acute Stroke “Diffusion Weighted Imaging (DWI) has proven to be the most effective means of detecting early strokes” Lehigh Magnetic Imaging Center Conventional T2 WI DW-EPI Sodium ion pumps fail, water goes in cells and can not diffuse.

  3. Tumor T2 (bright water) DWI (x direction) (T2 (bright water)+(diffusion)) T1 + Gadolinium

  4. Why MRI (more recently): Fiber Tracking

  5. 1st level of complexity Diffusion Weighted Image X direction • Higher diffusion in X direction  lower signal Artifact or Abnormality David Porter - November 2000

  6. Pulse Sequence: Gradient-EchoDiffusion Weighting in X direction Excitation 90o RF G -G Gx EPI (T2) Image Acquisition diffusiongradients Gy Gz

  7. Gradient Coils

  8. y Time RF Gx - Gy Gz x NO DIFFUISION protons (Hydrogen) with DIFFUISION signalloss

  9. Time T2 + diffusion T2 RF Gx - Gy Gz T2 (gradient strength)

  10. DIFFUSION MAPS weak (used to remove spin density, T1, T2, TR, and TE effects) S=S0e(-bDxx) Ln(S) = Ln(S0) – bDxx or Dxx = (Ln(S0) – Ln(S))/b Dxx Most Important image S (T2* EPI + weak Diffusion in X direction) S0(T2* EPI)

  11. 2nd Level of complexity DWI : 3 Direction • single-shot EPI diffusion-weighted (DW) images with b = 1000s/mm2 and diffusion gradients applied along three orthogonal directions • Higher diffusion  lower signal Dxx Dyy Dzz  courtesy of Dr Sorensen, MGH, Boston David Porter - November 2000

  12. PE FE SS A Little More Detail Y Diffusion-Weighting Z Diffusion-Weighting X Diffusion-Weighting y  x z GFE GPE GSS RF x y z

  13. X Diffusion-Weighting Z Diffusion-Weighting Y Diffusion Weighting Apparent Diffusion Coefficient ADC (AKA TRACE) used in clinical stroke, tumor, etc ADC = (Dxx + Dyy + Dzz)/3 Orientation independent No directional information (ie direction of greatest diffusion)

  14. 3rd level of complexityDiffusion Tensor Imaging Basics • Measures water diffusion in at least 6 directions – we use 12 • Echo-planar imaging (fast acquisition) • Collecting small voxels (1.8x1.8x3mm), scanning takes about 10 minutes

  15. Higher diffusion  lower signal • Useful for following white matter tracts

  16. Higher diffusion  lower signal Isotropic Anisotropic Adapted from: Beaulieu (2002). NMR in Biomed; 15:435-455

  17. DTI ellipsoidmeasure 6 directions to describe z y no diffusion x

  18. Ellipsoid Image Information available through DTI Tract Pierpaoli and Basser, Toward a Quantitative Assessment of Diffusion Anisotropy, Magn. Reson. Med, 36, 893-906 (1996)

  19. Tract tracing problems (fiber crossing)

  20. Tractography Superior view color fiber maps Lateral view color fiber maps Zhang & Laidlaw: http://csdl.computer.org/comp/proceedings/vis/2004/8788/00/87880028p.pdf.

  21. MRISC axial cor sag Diffusion Tensor Imaging data for cortical spinal tract on right side blue = superior – inferior fibers green = anterior – posterior fibers red = right – left fibers Note tumor is darker mass on left side of axial slice

  22. DxxDxyDxz DyxDyy Dyz Dzx DzyDzz But what is a diffusion tensor? It is a mathematical description of the ellipsoid. no diffusion xy -xy y-z xz z -xz y-z y z x

  23. Dxx Dxy Dxz Dyx Dyy Dyz Dzx Dzy Dzz Dx’x’ 0 0 0 Dy’y’ 0 0 0 Dz’z’ Whatisdiffusion“Tensor” (D)? difussion gradient direction vector y S=S0exp(-bD) labreferenceframe ellipsoidreferenceframe = (mathematical manipulation) Calculate FA (fractional anisotropy) Fiber track

  24. FA (fractional anisotropy) Information available through DTI Dy’y’ Dx’x’ Dz’z’ FA = ((Dx’x’-Dav)2 + (Dy’y’-Dav)2 + (Dz’z’-Dav)2)0.5 (Dx’x’2+Dy’y’2+Dz’z’2)0.5 s av FA = 0.9 FA = 0

  25. FA (fractional anisotropy) MAP

  26. FA + color(largest diffusion direction) red = right – left green = anterior – posterior blue = superior - inferior

  27. probably no use

  28. Tractography Superior view color fiber maps Lateral view color fiber maps Zhang & Laidlaw: http://csdl.computer.org/comp/proceedings/vis/2004/8788/00/87880028p.pdf.

  29. Signal loss : by intra-voxel phase dispersion At the echo time TE, NMR signal is decayed by, - T2 decay (spin-spin diffusion) - diffusive motion For any set of diff. gradient pulses G G 90 180 echo TE

  30. DTI Scalar Parameters • Trace: Magnitude of diffusion in a voxel. • Increases in damaged white matter • Fractional Anisotropy (FA): Measure of directionally-restricted diffusion. • Decreases in damaged white matter Rosenbloom M, et al. (July 2004). NIAA pubs; http://www.niaaa.nih.gov/publications/arh27-2/146-152.htm

  31. The Diffusion Tensor, D • Diffusion is not equal in all directions (anisotropic). • Use this to probe brain structure! • Represent the diffusion pattern at each point in the brain using an ellipsoid.

  32. Diffusion Vector (Colour) Map • The three magnitudes of the diffusion ellipsoid can be shown using three colours (RGB). • Red = Left – Right • Green = Ant. – Pos. • Blue = Sup. – Inf. • Map of major directions of water movement in the brain.

  33. Fibre Tractography • In principle, the locations of major white matter fibre tracts in the brain can be mapped using the information in the colour map, by “following the arrows.”

  34. Hindered Diffusion (diffusion ellipsoid) without hindrance WILSON with hindrance

  35. Information available through DTI – Orientation of λ1 • Useful for following white matter tracts

  36. Information available through DTI -- Aσ s av • Related to the shape of the ellipsoid • Independent of Dav (normalized) • Zero for a sphere, positive for other shapes • Sensitive to myelination and cortical development

  37. Diffusion Tensor Imaging (As) Normal Adult Brain (A maps)

  38. CELL EXTRA-CELLULAR SPACE FREELY DIFFUSING WATER IN EXTRA-CELLULAR SPACE • Higher diffusion  lower signal Tissue Sample A Tissue Sample B Freely Diffusing Water = Dark Larger D Restricted Diffusion = Bright Smaller D

  39. K-space view of the spin echo imaging Ky 1 2 3 . . . . . . . n Kx

  40. PE FE SS Diff. Grad. along different axis Y Diffusion-Weighting Z Diffusion-Weighting X Diffusion-Weighting GFE GPE GSS RF

  41. DTI (Diffusion Tensor Imaging) • single-shot EPI diffusion-weighted (DW) images with b = 1000s/mm2 and diffusion gradients applied along three orthogonal directions • Higher diffusion  lower signal  courtesy of Dr Sorensen, MGH, Boston David Porter - November 2000

  42. Detection of Acute Stroke “Diffusion Weighted Imaging (DWI) has proven to be the most effective means of detecting early strokes” Lehigh Magnetic Imaging Center Conventional T2 WI DW-EPI Sodium ion pumps fail, water goes in cells and can not diffuse.

  43. Tumor T2 (bright water) DWI (x direction) (T2 (bright water)+diffusion) T1 + Gadolinium

  44. Excitation 90o RF G -G Gx Image Acquisition Gy Gz Do X, Y , and Z at the same time

  45. y z x The Diffusion Tensor, D • Diffusion is not equal in all directions (anisotropic). • Use this to probe brain structure! • Diffusion ellipsoid for each voxel

  46. DTI Scalar Parameters • Trace: The magnitude of diffusion in a voxel. • Fractional Anisotropy (FA): The extent to which diffusion is directionally restricted.

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