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Selective and Differential Media

Selective and Differential Media. EMB. Selective for Gram negative E.coli has green metallic sheen. No growth of Gram pos Staph aureus. MSA: Selective and Differential. Selective because of salt: only Staph can grow.

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Selective and Differential Media

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  1. Selective and Differential Media

  2. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus

  3. MSA: Selective and Differential Selective because of salt: only Staph can grow Differential because S. aureus ferments mannitol sugar (turns it yellow) but Staph epidermidis does not ferment mannitol. Note there is no growth or fermentation from M. luteus.

  4. MSA with Staph aureus

  5. BLOOD AGAR: Enrichment media, and Differential

  6. Fish Eye Colonies of Enterobacter on blood agar

  7. MacConkey’s Agar:Selective and Differential Selective because Gram pos are inhibited by the bile salts. Differential because it has lactose. Gram negs that ferment lactose turn yellow.

  8. Antibiotic Sensitivity

  9. Antibiotic Sensitivity Organism is resistant to the antibiotic Organism is susceptible to the antibiotic

  10. Antibiotic Sensitivity: Check zone diameters on given table Organism has intermediate resistance to the antibiotic Organism is resistant to the antibiotic Organism is susceptible to the antibiotic

  11. Positive Coagulase Test:Differentiates S. Aureus from S epidermidis

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