Download

Tetrad analysis






Advertisement
/ 48 []
Download Presentation
Comments
jarrett
From:
|  
(124) |   (0) |   (0)
Views: 108 | Added:
Rate Presentation: 0 0
Description:
Tetrad analysis. Fig 5.15. Nonparental = recombinant . Tetrad analysis. What if two crossovers?. Four recombinants Two recombinants No recombinants. Fig 5.15. Parental ≠ non-recombinant. Fig. 5.17. Centromere is not a mutation. But you can still use it for mapping. Fig. 5.21.
Tetrad analysis

An Image/Link below is provided (as is) to

Download Policy: Content on the Website is provided to you AS IS for your information and personal use only and may not be sold or licensed nor shared on other sites. SlideServe reserves the right to change this policy at anytime. While downloading, If for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.











- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -




Slide 1

Tetrad analysis

Fig 5.15

Nonparental = recombinant

Slide 2

Tetrad analysis

What if two crossovers?

Four recombinants

Two recombinants

No recombinants

Fig 5.15

Slide 3

Parental ≠ non-recombinant

Fig. 5.17

Slide 4

Centromere is not a mutation

But you can still use it for mapping

Fig. 5.21

Slide 5

How do we know that radiation causes genetic defects?

Slide 6

Morgan

Bridges

Muller

Sturtevant

Slide 7

Muller’s X-ray experiment (1928)

Slide 8

Muller’s X-ray experiment

Created XClB

Slide 9

Muller’s X-ray experiment

Created XClB

XY

Slide 10

Muller’s X-ray experiment

Created XClB

•XY

gametes

Slide 11

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

Slide 12

Muller’s X-ray experiment

Created XClB

•XY x XClBX

(Bar-eyed females)

gametes

Slide 13

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

(ignore males)

Slide 14

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

Slide 15

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

XY x •XXClB

Slide 16

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

XY x •XXClB

•XY, XClBY

(ignore females)

Slide 17

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

XY x •XXClB

•XY, XClBY

(lethal)

Slide 18

Muller’s X-ray experiment

Created XClB

•XY x XClBX

gametes

•XX, •XXClB

XY x •XXClB

•XY, XClBY

How many lethal mutations?

(lethal)

Slide 19

Muller’s X-ray experiment

•XY, XClBY

How many lethal mutations?

(lethal)

Slide 20

Muller’s X-ray experiment

•XY, XClBY

How many lethal mutations?

(lethal)

Nobel prize to Muller, 1946: “for the discovery of the production of mutations by means of X-ray irradiation”

Slide 21

Important for radiation biology, but also for basic genetics

Slide 22

George Beadle & Edward Tatum (1941)

Do mutations affect biochemistry or only death, wing shape, pea color, etc?

Slide 23

Bread mold

Fig. 5.14

Slide 24

Beadle & Tatum

Complete med

Minimal med

Slide 25

Beadle & Tatum

(why?)

Complete med

Minimal med

Slide 26

Beadle & Tatum

Complete med

Minimal med

Complete med

Slide 27

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

Slide 28

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

Minimal + alanine

Slide 29

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

Minimal + serine

Slide 30

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

Minimal + B6

Slide 31

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

What’s wrong with the strain?

It can use everything but B6

It can make everything but B6

It can grow on everything but B6

Minimal + B6

Slide 32

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

(what is a vitamin?)

Minimal + B6

Slide 33

Beadle & Tatum

Complete med

Minimal med

Complete med

Minimal med

pdx strain

Minimal + B6

Slide 34

Beadle & Tatum

Slide 35

Beadle & Tatum

Slide 36

Beadle & Tatum

Now…how many mutations cause pdx?

Slide 37

Beadle & Tatum

pdx x WT

(Haploid)

(Haploid)

Slide 38

Beadle & Tatum

(if single-gene, what do we expect?)

pdx x WT

Slide 39

Beadle & Tatum

pdx x WT

Slide 40

Beadle & Tatum

pdx x WT

Slide 41

Beadle & Tatum

pdx x WT

Slide 42

Beadle & Tatum

pdx x WT

Slide 43

Beadle & Tatum

pdx x WT

Slide 44

Beadle & Tatum

Failure to make B6 segregates 1:1

Slide 45

Beadle & Tatum

Failure to make B6 segregates 1:1

so…

mutation in a single gene

Slide 46

Beadle & Tatum

Failure to make B6 segregates 1:1

so…

mutation in a single gene

so…

Step in B6 synthesis attributed to a single gene

“One-gene-one-enzyme”

Slide 47

Beadle & Tatum

Failure to make B6 segregates 1:1

so…

mutation in a single gene

so…

Nobel prize to Beadle & Tatum, 1958: “for their discovery that genes act by regulating definite chemical events”

Slide 48

Beadle & Tatum

Not quite one-gene-one-enzyme:

Fig. 9.1

“One-gene-one-polypeptide”


Copyright © 2014 SlideServe. All rights reserved | Powered By DigitalOfficePro