1 / 1

Gentile et al., Figure 1A

A. B. Gentile et al., Figure 1A. C. Gentile et al., Supplementary Figure 1

ganit
Download Presentation

Gentile et al., Figure 1A

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. A B Gentile et al., Figure 1A C Gentile et al., Supplementary Figure 1 Functional validation of the PGN triple fusion and design of PGN-containing trap vectors. (A) Average fluorescence, measured by flow cytometry, of MLP-29 cells stably transfected with GFP, GFNR (GFP-NTR) and PGN (PuroR-GFP-NTR). (B) FACS analysis of PGN-expressing cells after pharmacological selections. The histograms display the distribution of green fluorescence for four populations of cells: (i) untransduced cells (blue line); (ii) the starting population of MLP-29 cells stably transfected with PGN and treated with low doses of puromycin (1g/ml; black line and background); (iii) the low puromycin-selected cells further treated with high doses of puromycin (20g/ml; green line), or (iv) further treated with 10mM MN (red line). (C) Schematic view of P-TRACT, R-TRACT and L-TRACT, as indicated. SA, splice acceptor; pA, polyadenylation signal; , viral encapsidation signal, including the 5’ portion of gag gene (GA); RRE, rev responsive element, cPPT, central polypurine tract;. enh indicates that the 3’LTR enhancer is deleted.

More Related