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Propionic acid bacteria. Olivia Rasigraf HMC 2012. General. Gram + , non- sporeforming Facultative anaerobes High GC Secondary fermenters Contain cytochromes Habitats: skin, cheese. Isolation. Lactate-yeast extract broth. Incubation at 30°C, dark. “ Emmentaler ” habitat. CO 2.

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propionic acid bacteria

Propionic acid bacteria

Olivia Rasigraf

HMC 2012

general
General
  • Gram+, non-sporeforming
  • Facultative anaerobes
  • High GC
  • Secondary fermenters
  • Contain cytochromes
  • Habitats: skin, cheese
slide3

Isolation

Lactate-yeast extract broth

Incubation at 30°C, dark

emmentaler habitat
“Emmentaler” habitat

CO2

Lactobacillus casei

Streptococcus thermophilus

Propionibacteriumshermanii

3 Lactate → 2 Propionate + 1 Acetate + 1 CO2 + 1 ATP

methylmalonyl coa pathway
Methylmalonyl-CoA pathway

Lactate

Pyruvate

Acetyl-CoA

Acetyl-P

Acetate

Oxidative pathway

methylmalonyl coa pathway1
Methylmalonyl-CoA pathway

Pyruvate

Acetyl-CoA

Acetyl-P

Lactate

Acetate

Oxalacetate

Propionate

Malate

Reductive pathway

Fumarate

Succinate

Methylmalonyl-CoA mutase (B12)

acrylyl coa pathway
Acrylyl-CoA pathway

Acetate

Acetyl-P

Pyruvate

Lactate

Propionate

results
Results
  • All enrichments successful
  • Isolation of single colonies

Brown colonies: Methylmalonyl-CoA pathway

White colonies: Acrylyl pathway

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