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Propionic acid bacteria. Olivia Rasigraf HMC 2012. General. Gram + , non- sporeforming Facultative anaerobes High GC Secondary fermenters Contain cytochromes Habitats: skin, cheese. Isolation. Lactate-yeast extract broth. Incubation at 30°C, dark. “ Emmentaler ” habitat. CO 2.

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Propionic acid bacteria

Propionic acid bacteria

Olivia Rasigraf

HMC 2012


General
General

  • Gram+, non-sporeforming

  • Facultative anaerobes

  • High GC

  • Secondary fermenters

  • Contain cytochromes

  • Habitats: skin, cheese


Isolation

Lactate-yeast extract broth

Incubation at 30°C, dark


Emmentaler habitat
Emmentaler” habitat

CO2

Lactobacillus casei

Streptococcus thermophilus

Propionibacteriumshermanii

3 Lactate → 2 Propionate + 1 Acetate + 1 CO2 + 1 ATP


Methylmalonyl coa pathway
Methylmalonyl-CoA pathway

Lactate

Pyruvate

Acetyl-CoA

Acetyl-P

Acetate

Oxidative pathway


Methylmalonyl coa pathway1
Methylmalonyl-CoA pathway

Pyruvate

Acetyl-CoA

Acetyl-P

Lactate

Acetate

Oxalacetate

Propionate

Malate

Reductive pathway

Fumarate

Succinate

Methylmalonyl-CoA mutase (B12)


Acrylyl coa pathway
Acrylyl-CoA pathway

Acetate

Acetyl-P

Pyruvate

Lactate

Propionate


Results
Results

  • All enrichments successful

  • Isolation of single colonies

Brown colonies: Methylmalonyl-CoA pathway

White colonies: Acrylyl pathway




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