An evaluation study for fibre analysis by uv vis microspectrophotometry
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AN EVALUATION STUDY FOR FIBRE ANALYSIS BY UV-VIS MICROSPECTROPHOTOMETRY. By Johanne Almer, Eleanor McAnsh, & Barbara Doupe. Centre of Forensic Sciences Ministry of Community Safety and Correctional Services, Ontario . INTRODUCTION.

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An evaluation study for fibre analysis by uv vis microspectrophotometry

AN EVALUATION STUDY FOR FIBRE ANALYSIS BY UV-VIS MICROSPECTROPHOTOMETRY

By Johanne Almer, Eleanor McAnsh, & Barbara Doupe

Centre of Forensic Sciences Ministry of Community Safety and Correctional Services, Ontario


Introduction
INTRODUCTION MICROSPECTROPHOTOMETRY

Colour can be a highly discriminating feature in forensic fibre comparison.

VIS microspectrophotometry increased the ability for colour discrimination.

Further discrimination can be obtained in the UV spectral range.


Purpose
PURPOSE MICROSPECTROPHOTOMETRY

In this study, various aspects of UV-VIS microspectrophotometry were assessed to evaluate its use in a forensic laboratory for routine fibre analysis.


Purpose1
PURPOSE MICROSPECTROPHOTOMETRY

  • Instrumental stability

  • Interferences from mounting materials

  • Interferences from fibre polymers

  • Its relative value compared to VIS microspectrophotometry


Methodology instrumental stability
METHODOLOGY MICROSPECTROPHOTOMETRYInstrumental stability

Same-day fibre analysis reproducibility

  • Green acrylic fibre absorbance was measured 6x in approx the same location over 8 hour period

    Day-to-day fibre analysis reproducibility

  • Green acrylic fibre absorbance was measured 6x in approx the same location over 28 day period


Results instrumental stability
RESULTS MICROSPECTROPHOTOMETRYInstrumental stability

Spectral results from same-day


Results instrumental stability1
RESULTS MICROSPECTROPHOTOMETRYInstrumental stability

Spectral results from day-to-day


Methodology interferences from mounting materials
METHODOLOGY MICROSPECTROPHOTOMETRYInterferences from mounting materials

UV-VIS absorbance was measured through:

  • Glass slide

  • Quartz slide

  • Glycerine on quartz

  • XAM on quartz


Results interferences from mounting materials
RESULTS MICROSPECTROPHOTOMETRYInterferences from mounting materials

Absorbance spectra of mounting materials


Methodology interferences from fibre polymers
METHODOLOGY MICROSPECTROPHOTOMETRYInterferences from fibre polymers

UV-VIS absorbance was measured for white, non-dyed fibres of different polymer types:

  • Cotton, ramie, wool, silk

  • Di-acetate, triacetate, viscose

  • Nylon 6, nylon 66, polyethylene, polypropylene, PET, PCDT

  • Modacrylics: PAN/VC, PAN/VDC, PAN/VDC/MMA, PAN/VDC/VBr/ARS

  • Acrylics: PAN/MA, PAN/MMA, PAN/VA, PAN/PVP, PAN/MA/MVP, PAN/VA/MVP


Results interferences from fibre polymers
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

  • High UV absorbance of wool, silk, polyester, and acrylics with MVP

  • Moderate to low UV absorbance of acetates, nylons, modacrylics and acrylics with PVP

  • No UV absorbance of cotton, ramie, viscose, olefin and acrylic with no PVP/MVP


Results interferences from fibre polymers1
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Protein fibres


Results interferences from fibre polymers2
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Polyester fibres


Results interferences from fibre polymers3
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Polyamide fibres


Results interferences from fibre polymers4
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Cellulosic fibres


Results interferences from fibre polymers5
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Acrylic fibres


Results interferences from fibre polymers6
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Modacrylic fibres


Results interferences from fibre polymers7
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Modacrylic fibres


Results interferences from fibre polymers8
RESULTS MICROSPECTROPHOTOMETRYInterferences from fibre polymers

Olefin fibres


Methodology vis analysis vs uv vis analysis
METHODOLOGY MICROSPECTROPHOTOMETRYVIS analysis vs UV-VIS analysis

  • Absorbance was measured between 240-770nm with coloured fibres mounted on quartz and on glass, and

  • Absorbance was measured between 380-770nm with coloured fibres mounted on glass


Methodology vis analysis vs uv vis analysis1
METHODOLOGY MICROSPECTROPHOTOMETRYVIS analysis vs UV-VIS analysis

  • Wool: red

  • Viscose: yellow, orange, red, pink, purple, green, blue, brown, and black

  • Di-acetate: yellow, tangerine, red, pink, violet, green, blue, brown, and black

  • Nylon 66: yellow, red burgundy, aqua, blue, brown, and black

  • Acrylic PAN/MMA: yellow, orange, rust, fuschia, purple, green, blue, brown, and charcoal


Results vis analysis vs uv vis analysis
RESULTS MICROSPECTROPHOTOMETRYVIS analysis vs UV-VIS analysis

  • Peak shapes were the same in the visible range

  • No trend in sensitivity


Results fibre polymers with high uv absorbance
RESULTS MICROSPECTROPHOTOMETRYFibre polymers with high UV absorbance

Red wool fibre


Results fibre polymers with low uv absorbance
RESULTS MICROSPECTROPHOTOMETRYFibre polymers with low UV absorbance

Violet and white di-acetate fibres


Results fibre polymers with no uv absorbance
RESULTS MICROSPECTROPHOTOMETRYFibre polymers with no UV absorbance

Orange viscose fibre


Results fibre polymers with no uv absorbance1
RESULTS MICROSPECTROPHOTOMETRYFibre polymers with no UV absorbance

Rust acrylic fibre


Conclusions
CONCLUSIONS MICROSPECTROPHOTOMETRY

The microspectrophotometry of all coloured fibres can initially be done with XAM-mounted glass slides from 300-770nm with the UV-VIS system.

  • Additional peaks can be obtained for all fibres by extending the range to include 300-380nm.


Conclusions1
CONCLUSIONS MICROSPECTROPHOTOMETRY

The need for re-mounting onto quartz slides for full UV-VIS analysis at 240-770nm depends on the fibre type and spectral results.

  • For fibres which have a increasing slope below 300nm need to be re-mounted.

  • For fibres which have no increasing slope below 300nm should be re-mounted.

  • For fibres which have polymers with high UV absorbance no re-mounting would be necessary.


Conclusions2

Fibres Mounted on Glass Slides MICROSPECTROPHOTOMETRY

Analyse Fibres

300-770nm

Analyse Fibres

300-770nm

Done

No

Yes

Re-analyse Fibres on Quartz Slides

CONCLUSIONS

Nylons, Acetates, Modacrylic, Cotton, Ramie, Viscose, All other Acrylics, and Olefins

Silk, Wool, Acrylic with MVP, and Polyester

Absorbance at 300nm

> non-dyed referencepolymer spectra?


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