Culturing bioluminescent bacteria
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culturing bioluminescent bacteria. dead fish, i love you, but you’re bringing me down. b acterial bioluminescence. Is interesting Diverse Vibrios in the sea Goal: to isolate bioluminescent bacteria for further study. strategy.

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Culturing bioluminescent bacteria

culturing bioluminescent bacteria

dead fish,

i love you,

but you’re bringing me down


B acterial bioluminescence

bacterial bioluminescence

  • Is interesting

  • Diverse Vibriosin the sea

  • Goal: to isolate bioluminescent bacteria for further study


Strategy

strategy

  • Bioluminescent bacteria are present throughout marine environments

  • Bioluminescence is detectable when cells are present at high density

  • Therefore: make bacteria grow to induce luminescence, pick them out


Culturing bioluminescent bacteria

Seawater complete media

  • Artificial seawater, bactotryptone, yeast extract, glycerol, agar


Culturing bioluminescent bacteria

Photo courtesy S. Russell


How do we improve yields

How do we improve yields?

  • Sample from enriched locations

  • Improve culturability (great plate count anomaly)

    • In situ culturing with diffusion chambers?

D’Onofrio et al (2010) Chemistry & Biology. 17(3):256-264


How to improve yields cont d

How to improve yields? (cont’d)

  • More selective media, or bait?

    • chemotaxis

    • Non-symbiosis-related functions of bioluminescence?

  • Improve detection on plate


Culturing bioluminescent bacteria

the end


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