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culturing bioluminescent bacteria. dead fish, i love you, but you’re bringing me down. b acterial bioluminescence. Is interesting Diverse Vibrios in the sea Goal: to isolate bioluminescent bacteria for further study. strategy.

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culturing bioluminescent bacteria

culturing bioluminescent bacteria

dead fish,

i love you,

but you’re bringing me down

b acterial bioluminescence
bacterial bioluminescence
  • Is interesting
  • Diverse Vibriosin the sea
  • Goal: to isolate bioluminescent bacteria for further study
strategy
strategy
  • Bioluminescent bacteria are present throughout marine environments
  • Bioluminescence is detectable when cells are present at high density
  • Therefore: make bacteria grow to induce luminescence, pick them out
slide4

Seawater complete media

  • Artificial seawater, bactotryptone, yeast extract, glycerol, agar
how do we improve yields
How do we improve yields?
  • Sample from enriched locations
  • Improve culturability (great plate count anomaly)
    • In situ culturing with diffusion chambers?

D’Onofrio et al (2010) Chemistry & Biology. 17(3):256-264

how to improve yields cont d
How to improve yields? (cont’d)
  • More selective media, or bait?
    • chemotaxis
    • Non-symbiosis-related functions of bioluminescence?
  • Improve detection on plate
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