my experiment c o l o r c h r o m a t o g r a p h y
Download
Skip this Video
Download Presentation
My Experiment C o l o r C h r o m a t o g r a p h y

Loading in 2 Seconds...

play fullscreen
1 / 20

My Experiment C o l o r C h r o m a t o g r a p h y - PowerPoint PPT Presentation


  • 93 Views
  • Uploaded on

My Experiment C o l o r C h r o m a t o g r a p h y. Presented By Kelsey Leigh Campbell Date: 3 October 2012. What is Chromatography?. Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' My Experiment C o l o r C h r o m a t o g r a p h y' - eadoin


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
my experiment c o l o r c h r o m a t o g r a p h y

My ExperimentColorChromatography

Presented By

Kelsey Leigh Campbell

Date: 3 October 2012

what is chromatography
What is Chromatography?

Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

uses for chromatography
Uses for Chromatography
  • Analyze
  • Identify
  • Purify
  • Quantify

Chromatography is used by scientists to

uses for chromatography1
Uses for Chromatography
  • Pharmaceutical Company
  • Hospital
  • Law Enforcement
  • Environmental Agency
  • Manufacturing Plant

Real-life examples of uses for chromatography

chromatography defined
Chromatography Defined

Detailed Definition:

Chromatography is a laboratory technique that separates components within a mixture by using the differential affinities of the components for a mobile medium and for a stationary absorbing medium through which they pass

types of chromatography
Types of Chromatography
  • Liquid Chromatography
  • Gas Chromatography
  • Paper Chromatography
  • Thin-Layer Chromatography
principles of paper chromatography
Principles of Paper Chromatography
  • Capillary Action – the movement of liquid within the spaces of a porous material due to the forces of adhesion, cohesion, and surface tension.
  • Solubility – the degree to which a material (solute) dissolves into a solvent.
overview of the experiment
Overview of the Experiment

Purpose

To introduce students to the principles and terminology of chromatography and demonstrate separation of the dyes in Sharpies with paper chromatography

Time Required

Prep Time: 20 minutes

Experiment: 45 minutes

materials list
Materials List
  • 6 strips of paper or filter
  • 6 beakers or jars
  • All strips must be exactly the same size (e.g., 3 cm × 9 cm)
  • Different color Sharpies
  • Volumetric measuring devices
  • Water
  • Isopropanol
  • Pencil
  • Ruler
  • Tape
preparation
Preparation
  • Prepare 15 ml of the following isopropanol solutions in appropriately labeled beakers:
    • 0%, 20%, 50%, 70%, 100%
  • Cut 5 strips of filter paper
  • Draw a line 1 cm above the bottom edge of the strip with the pencil
  • Label each strip with its corresponding solution (color and concentration)
  • Place a spot from each marker (or other dye sample) on your starting line
developing the chromatograms
Developing the Chromatograms
  • Place the strips in the beakers
  • Make sure the solution does not come above your start line
  • Keep the beakers covered
  • Let strips develop until the ascending solution front is about 2 cm from the top of the strip
  • Remove the strips and let them dry
observing the chromatograms
Observing the Chromatograms
  • Observe how some of the dyes are made up of more than one color
  • Observe how spots of the same color separated in low concentrations of alcohol compared to higher concentrations
  • Observe when spots of different colors first started separating in the different concentrations
slide15

0 %

20 %

50 %

70 %

100 %

black and blue s e p a r a t i o n s
Black and Blue Separations
  • Black Dye
    • Dyes Separated: black and purple
    • Not soluble in low concentrations of isopropanol
    • Partially soluble in concentrations >20%
  • Blue Dye
    • Dye Separated: blue
    • Not very soluble in low concentrations
    • Completely soluble in high concentrations
green and red s e p a r a t i o n s
Green and Red Separations
  • Green Dye
    • Dyes Separated: Blue and Yellow
    • Blue: Soluble in concentrations > 20%
    • Yellow: Soluble in concentrations > 0%
  • Red Dye
    • Dyes Separated: Red and Yellow
    • Yellow: Soluble in low concentrations and less soluble in high concentrations
    • Red: Slightly soluble in low concentrations and more soluble in concentrations > 20%
alternative experiments
Alternative Experiments
  • Test Different Samples
    • Markers, pens, highlighters
    • Food pigments (Skittles or M&M’s)
  • Test Different Solvents
    • Other alcohols: methanol, ethanol, propanol, butanol
  • Test Different Papers
    • Coffee Filters
    • Paper towels
    • Typing Paper
references
References
  • The idea for this experiment came from http://chemistry.about.com/od/chemistryexperiments/ht/candychroma.htm
  • Scientific Uses for Chromatography

http://www.wjec.co.uk/uploads/publications/5403.pdf

  • Everyday Uses for Chromatography

http://www.chacha.com/question/what-are-two-practical-uses-of-chromatography-in-everyday-life

http://www.blurtit.com/q6751913.html

ad