My experiment c o l o r c h r o m a t o g r a p h y
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My Experiment C o l o r C h r o m a t o g r a p h y. Presented By Kelsey Leigh Campbell Date: 3 October 2012. What is Chromatography?. Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.

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My experiment c o l o r c h r o m a t o g r a p h y

My ExperimentColorChromatography

Presented By

Kelsey Leigh Campbell

Date: 3 October 2012


What is chromatography

What is Chromatography?

Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components.


Uses for chromatography

Uses for Chromatography

  • Analyze

  • Identify

  • Purify

  • Quantify

Chromatography is used by scientists to


Uses for chromatography1

Uses for Chromatography

  • Pharmaceutical Company

  • Hospital

  • Law Enforcement

  • Environmental Agency

  • Manufacturing Plant

Real-life examples of uses for chromatography


Chromatography defined

Chromatography Defined

Detailed Definition:

Chromatography is a laboratory technique that separates components within a mixture by using the differential affinities of the components for a mobile medium and for a stationary absorbing medium through which they pass


Chromatography illustration

Chromatography Illustration


Types of chromatography

Types of Chromatography

  • Liquid Chromatography

  • Gas Chromatography

  • Paper Chromatography

  • Thin-Layer Chromatography


Principles of paper chromatography

Principles of Paper Chromatography

  • Capillary Action – the movement of liquid within the spaces of a porous material due to the forces of adhesion, cohesion, and surface tension.

  • Solubility – the degree to which a material (solute) dissolves into a solvent.


Overview of the experiment

Overview of the Experiment

Purpose

To introduce students to the principles and terminology of chromatography and demonstrate separation of the dyes in Sharpies with paper chromatography

Time Required

Prep Time: 20 minutes

Experiment: 45 minutes


Materials list

Materials List

  • 6 strips of paper or filter

  • 6 beakers or jars

  • All strips must be exactly the same size (e.g., 3 cm × 9 cm)

  • Different color Sharpies

  • Volumetric measuring devices

  • Water

  • Isopropanol

  • Pencil

  • Ruler

  • Tape


Preparation

Preparation

  • Prepare 15 ml of the following isopropanol solutions in appropriately labeled beakers:

    • 0%, 20%, 50%, 70%, 100%

  • Cut 5 strips of filter paper

  • Draw a line 1 cm above the bottom edge of the strip with the pencil

  • Label each strip with its corresponding solution (color and concentration)

  • Place a spot from each marker (or other dye sample) on your starting line


Developing the chromatograms

Developing the Chromatograms

  • Place the strips in the beakers

  • Make sure the solution does not come above your start line

  • Keep the beakers covered

  • Let strips develop until the ascending solution front is about 2 cm from the top of the strip

  • Remove the strips and let them dry


Observing the chromatograms

Observing the Chromatograms

  • Observe how some of the dyes are made up of more than one color

  • Observe how spots of the same color separated in low concentrations of alcohol compared to higher concentrations

  • Observe when spots of different colors first started separating in the different concentrations


My experiment c o l o r c h r o m a t o g r a p h y

0 %

20 %

50 %

70 %

100 %


Black and blue s e p a r a t i o n s

Black and Blue Separations

  • Black Dye

    • Dyes Separated: black and purple

    • Not soluble in low concentrations of isopropanol

    • Partially soluble in concentrations >20%

  • Blue Dye

    • Dye Separated: blue

    • Not very soluble in low concentrations

    • Completely soluble in high concentrations


Green and red s e p a r a t i o n s

Green and Red Separations

  • Green Dye

    • Dyes Separated: Blue and Yellow

    • Blue: Soluble in concentrations > 20%

    • Yellow: Soluble in concentrations > 0%

  • Red Dye

    • Dyes Separated: Red and Yellow

    • Yellow: Soluble in low concentrations and less soluble in high concentrations

    • Red: Slightly soluble in low concentrations and more soluble in concentrations > 20%


Alternative experiments

Alternative Experiments

  • Test Different Samples

    • Markers, pens, highlighters

    • Food pigments (Skittles or M&M’s)

  • Test Different Solvents

    • Other alcohols: methanol, ethanol, propanol, butanol

  • Test Different Papers

    • Coffee Filters

    • Paper towels

    • Typing Paper


My experiment c o l o r c h r o m a t o g r a p h y

Questions?!


References

References

  • The idea for this experiment came from http://chemistry.about.com/od/chemistryexperiments/ht/candychroma.htm

  • Scientific Uses for Chromatography

    http://www.wjec.co.uk/uploads/publications/5403.pdf

  • Everyday Uses for Chromatography

    http://www.chacha.com/question/what-are-two-practical-uses-of-chromatography-in-everyday-life

    http://www.blurtit.com/q6751913.html


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