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Microscope

Microscope. Basics. T. Trimpe 2005 http://sciencespot.net/. Base. -Single piece of metal -Support here when moving Color RED. Arm. -Hold here when moving Color Dark GREEN. Stage. -Supports Specimen -Hole allows light to pass through  Color BLUE. Diaphragm.

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Microscope

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  1. Microscope Basics T. Trimpe 2005 http://sciencespot.net/

  2. Base -Single piece of metal -Support here when moving Color RED

  3. Arm -Hold here when moving Color Dark GREEN

  4. Stage -Supports Specimen -Hole allows light to pass through  Color BLUE

  5. Diaphragm -controls amount of light -ranges 1-5 Color LIGHT GREEN

  6. Stage Clips -secures specimen/slide  Color GREY

  7. Bulb/Mirror -light source -NEVER use the sunlight it will hurt your eyes!! Color YELLOW

  8. Scanning Objective Lens -shortest lens -4x magnification Color BLACK

  9. Low Power Objective Lens -10x magnification Color PURPLE

  10. High Power Objective Lens -40x magnification Color ORANGE STRIPES

  11. Body Tube -allows light from objective to pass upward Color BROWN

  12. Eye Piece/Ocular lens -Site of observation -10x magnification Color ORANGE

  13. Nose Piece -Allows you to switch objective lenses Color BLUE and GREEN stripes

  14. Coarse Adjustment Knob -used to focus specimen -DO NOT use on high power objective lens Color PINK

  15. Fine Adjustment Knob -Used for minute focusing -Used after coarse adjustment knob Color Tan

  16. Total magnification = (ocular lens power) x (objective lens power) • Example: (10x)oculear lens x (40x)high power lens = 400x total magnification • Magnification: The ability to increase the size of an image X = 400x total magnification Objective Lens Ocular Lens

  17. Light Pathway: Light Source→Specimen→Objective Lens→Body Tube→Eye Piece

  18. Resolution • making the image clear • ability to determine between two objects or points • can be adjusted with the fine/course knob or the diaphragm (to allow more light in)

  19. We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification? Comparing Powers of Magnification

  20. Use of a Scope • Carry the microscope with 2 hands (arm & base) • Use closest electrical outlet without going through a walkway • Looking at a slide: • Use ONLY ONE slide at a time • ALWAYS start at LOW power first(10x) • ALWAYS start with the stage lowered completely • Make sure the objective clicks into position • The course focus knob is ONLY used on the lowest power lens • The fine focus knob is used on all lenses to bring the image into focus

  21. Putting the Microscope Away • Lower the stage completely and click the lowest objective into place • Return slide to tray • Wind up the scope power cord

  22. Agenda • Finish Microscope Handout • Glue in notebook when finished • Due tomorrow • Study Guide • Answer on separate piece of paper • Due Friday • Extra Credit Flashcards • Make 20 • Due Friday

  23. 2. Electron microscope: Uses electrons instead of light to visualize the specimen, electrons bounce off or pass through and a computer interprets a picture Always black and white, specimen must be dead to examine Scanning EM – magnifies up to 100,000x Transmission EM – magnifies up to 200,000x Types of Microscopes • 1. Compound Light Microscope: Shines light through a specimen and uses 2 lenses for magnification • Magnification – increase in an objects apparent size • Resolution – how clear the image appears once magnified • Max clear magnification ~2000x

  24. Microscope Images Compound Light Microscope - Protozoa Scanning Electron Microscope (SEM) Butterfly Tongue Transmission Electron Microscope - Bacteria

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