1 / 27

Gene Expression

Gene Expression. AKA Molecular Biology. Gene Expression Le Dogma Centrale. Genome Organization. Gene Structure. Transcription. Post-transcriptional Modification. Translation. Post-translational Modification. Regulatory Sequences. Transcription.

dennis
Download Presentation

Gene Expression

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gene Expression AKA Molecular Biology

  2. Gene ExpressionLe Dogma Centrale

  3. Genome Organization

  4. Gene Structure

  5. Transcription

  6. Post-transcriptional Modification

  7. Translation

  8. Post-translational Modification

  9. Regulatory Sequences

  10. Transcription • Just 3 little words that aren't that hard to say: • Initiation • Elongation • Termination

  11. RNA Polymerases

  12. RNA Polymerases

  13. Pol I Promoters

  14. Pol I Initiation

  15. Pol II Promoters

  16. Pol II Initiation

  17. Classes of mRNA • Abundant Class – Simple mRNA Fraction • 1- 5 different genes (transcripts) which represent >50% of total mRNA expressed in cell/tissue • 1,000 - 100,000 copies of each mRNA/cell • Moderate Class • 5-50 different genes (transcripts) which represent 10-20% of total mRNA expressed in cell/tissue • 10 -100 copies of each mRNA/cell • Rare Class – Complex mRNA Fraction • >100 different genes (usually 10-20K genes) which represent 10%-20% of the total mRNA expressed in a cell/tissue • 1-10 copies of each mRNA/cell

  18. Pol III Promoters

  19. Pol III Initiation

  20. Functional Domains of Eukaryotic Transcription Factors • DNA binding domain – DBD • Binds specific sequence of base pairs • Transcriptional activation domain – TAD • Interacts with basal txn factors or directly with RNA polymerase II • Protein-protein interaction domain – PPID • Homo- & Hetero dimerization domains • Interaction with other txn factors (besides the basal apparatus)

  21. Transcription Factor Families Family Representative Homeodomain Hox Hoxa-1, Antp, eve, en POU Pit-1,Unc-86, Oct-2, Brn-5 LIM Lim-1, Isl-1 Paired Box Pax-1,2,6, Prd Forkhead/Winged Helix Fkh, HNF-3 Basic helix-loop-helix MyoD, Ac, da, emc AP1, Myc, C/EBP leucine-zipper Zinc-finger monomeric WT-1, Krox-20, Kr, hb steroid receptors GR, ER, RAR, kni Sox Sry, SoxD, Sox2 MADS box MEF-1, MCM-1, AG, DEF, SRF

  22. Homeodomain First Identified in Genes Causing Homeotic Transformations Antennapedia Ultrabithorax

  23. Regulation of Gene Specific Transcription Factors

  24. Regulation of Gene Specific Transcription Factors

  25. Regulatory Hierarchies

More Related