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Project Wise 2004. Biotechnology : Performing the Technique of Gel Electrophoresis. James Rules! . James is a biological all-star! . What is Electrophoresis ?. I wonder if they will find out I killed the lab tech. Bill Ryan is next!.

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Project Wise 2004

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Project wise 2004 l.jpg

Project Wise 2004

Biotechnology:

Performing the Technique of Gel Electrophoresis

James Rules!

James is a biological all-star! 


What is electrophoresis l.jpg

What is Electrophoresis?

I wonder if they will find out I killed the lab tech.

Bill Ryan is next!

  • The separation of biological macromolecules in an electrical field.

  • Separation is based on:

    • Charge

    • Shape

    • Size

  • Electrophoresis has many, many uses

    • DNA Fingerprinting, Sequencing.

    • It is used for catching the bad guy/gal

      • C.S.I.


Lab 1 edvotek 102 restriction enzyme cleavage of dna l.jpg

Lab 1: EDVOTEK #102:Restriction Enzyme Cleavage of DNA

  • In this lab we used restriction enzymes in order to cut DNA at specific areas.


Lab 2 edvotek 104 size determination of dna restriction fragments l.jpg

Lab 2: EDVOTEK #104:Size Determination of DNA Restriction Fragments


Lab 3 edvotek 105 mapping of restriction sites on plasmid dna l.jpg

Lab 3: EDVOTEK #105Mapping of Restriction Sites on Plasmid DNA

  • The purpose of this lab is to accurately locate the genes on the chromosome.

  • This is used in the Human Genome Project.


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Have a Great End to Your Summer!

Project Wise Bio-Tech Group:

Laurie Asermily

Sheri Backus

Joe Bonanno

Davette Carpenter

Amber Corbin

Elaine Esan

Shawn Flanagan

Vonda McLeon

Jane O’Donnell

Steve Polo

Beth Shea


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