'Restriction sites' presentation slideshows

CHAPTER 18 Microbial Models: The Genetics of Viruses and Bacteria

CHAPTER 18 Microbial Models: The Genetics of Viruses and Bacteria. Size Averages Eukaryotic 10 um. Bacteria 1 um. Viruses .1um. Respiratory infections & pink eye. The human Papilloma virus causes warts and cervical cancer double stranded circular DNA.

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Biotechnology

Biotechnology. Biotechnology. The use of microorganisms, or biological substances, such as enzymes, to perform specific industrial or manufacturing processes. Science Fact or Fiction?. http://www.flickclip.com/flicks/jurassicpark3.html. Genetic Engineering. The process of producing

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Practical Synthetic Biology

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Molecular cloning

Lecture 6. Molecular cloning. With thanks to David Tscharke @ RSB. Lecture overview. AKA gene cloning or DNA cloning The generation of identical copies of a piece of DNA Propagated in bacteria that originate from a single cell Was the first recombinant DNA technology

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GENETIC TECHNOLOGY

GENETIC TECHNOLOGY. HONORS BIOLOGY 2A Motzko. Constantine Fahlberg - Saccharin (1879). James Schlatter - Aspartame (1965). Percy Spencer -Microwave Oven (1945). John Harvey Kellogg - Corn Flakes (1899). Alexander Fleming - Penicillin (1927).

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Terminology

Terminology. Branches, splits, bipartitions In a rooted tree: clades Mono-, Para-, polyphyletic groups, cladists and a natural taxonomy.

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Perl for Bioinformatics

Perl for Bioinformatics. Lecture 4. Variables - review. A variable name starts with a $ It contains a number or a text string Use my to define a variable Use = to assign a value Use to stop the variable being interpolated

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Ghosh Lab University of Arizona Department of Chemistry

Cloning 101: A Primer. Ghosh Lab University of Arizona Department of Chemistry. Outline. Cloning overview pDRAW32 Design Gene Insert Primers Further considerations (optimization of the process) Transformation. Cloning Overview. Four main steps in cloning: Insert synthesis

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AP Biology 12 Concept 2: Analyzing and utilizing biotechnology tools

AP Biology 12 Concept 2: Analyzing and utilizing biotechnology tools. Please refer to: Chapter 20 in Campbell Pg 136-141 in Holtzclaw Pg ______ in Holtzclaw Practice Questions: Campbell: #1-4 p. 405 (20.1) #1-3 p. 411 (20.2) #1-3 p. 416 (20.3)

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Cloning Human Taste Receptor Gene TAS1R3 and Miraculin

Cloning Human Taste Receptor Gene TAS1R3 and Miraculin. Team Chameleon (Jack and Kelsey). The Prequel. Miraculin is a taste-altering protein already present in the BioBricks library No one has been able to test if they have successfully produced functional miraculin ... UNTIL NOW!.

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Restriction Mapping

Restriction Mapping. Central Dogma. Human Migration. Gel Electrophoresis. Restriction Enzymes : Cut at specific and unique sequences called Restriction Sites. Restriction Site Mapping Like Solving a puzzle. Boreal Toads Bufo boreas boreas. Learning Goals.

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Enzymology of Recombinant DNA [Summary]

Enzymology of Recombinant DNA [Summary]. Jiyoun Lee. Enzyme. Definition A protein that speeds up chemical reactions in the body.

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Restriction Enzyme Cleavage of DNA

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Structural Genomics of Parasitic Protozoans ( Trypanosoma, Leishmania ) (SGPP East)

Structural Genomics of Parasitic Protozoans ( Trypanosoma, Leishmania ) (SGPP East). Protein Expression at the University of Rochester Mark Dumont, Elizabeth Grayhack. Eric Phizicky. Ligation-Independent Cloning (Uses PCR-vector overlap sequences missing one type of nucleotide).

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Primer design for PCR cloning

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Mystery Plasmid B ??????

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Chassis

Chassis. Parts jukebox : Meta-device : few strains Focus of system designs & integration Standardization, composability, abstraction Interface with environment, power supply Interface with computers: CAD, measurements Human practice: Safety & process engineering. Chassis Goals.

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RFLP

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Project Wise 2004

Project Wise 2004. Biotechnology : Performing the Technique of Gel Electrophoresis. James Rules! ?. James is a biological all-star! ?. What is Electrophoresis ?. I wonder if they will find out I killed the lab tech. Bill Ryan is next!.

190 views • 7 slides