Explore effective way to increase acid resistance of
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Explore effective way to increase acid resistance of lactococcus lactis F44 for higher production of nisin. Reporter: 冯文婧. Background. Nisin is a 34-amino acid antimicrobial peptide with various unusual amino acids and five ring structures. Broad host spectrum. easily being digested.

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Reporter: 冯文婧

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Reporter

Explore effective way to increase acid resistance of lactococcus lactis F44 for higher production of nisin

Reporter: 冯文婧


Reporter

Background

  • Nisin is a 34-amino acid antimicrobial peptide with various unusual amino acids and five ring structures


Reporter

Broad host

spectrum

easily being

digested

preservative


Reporter

limitation for higher production

Non-appropriate pH value

Nisin degrades quickly when pH value higher than 4.0 (data not shown)

Degradation from Protease

The contradiction between the optimum

growth pH (7.2) of L.lactis and stability of

nisin(pH2.0).

Improve acid resistance of L.lactis by transforming acid tolerance genes


Reporter

Review about acid resistance genes


Reporter

Overexpressing genes underlined in blue circle, to verify the influence

these exert on nisin production


Reporter

Screening for genes based on nisin production


Reporter

Our goal


Reporter

Thank You !


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