Explore effective way to increase acid resistance of lactococcus lactis F44 for higher production of nisin. Reporter: 冯文婧. Background. Nisin is a 34-amino acid antimicrobial peptide with various unusual amino acids and five ring structures. Broad host spectrum. easily being digested.
Explore effective way to increase acid resistance of lactococcus lactis F44 for higher production of nisin
limitation for higher production
Non-appropriate pH value
Nisin degrades quickly when pH value higher than 4.0 (data not shown)
Degradation from Protease
The contradiction between the optimum
growth pH (7.2) of L.lactis and stability of
Improve acid resistance of L.lactis by transforming acid tolerance genes
Review about acid resistance genes
Overexpressing genes underlined in blue circle, to verify the influence
these exert on nisin production
Screening for genes based on nisin production
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