Rapid identification of yeasts
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Rapid Identification of Yeasts. Rapid Identification. Two Alternative Approaches. Aim and Methodology. Topics to be covered. Saccharomyces species-specific. 2011. Discovery of S. eubayanus*. S. eubayanus species-specific primers have been developed (228bp).

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Rapid Identification of Yeasts

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Rapid identification of yeasts

Rapid Identification of Yeasts


Rapid identification

Rapid Identification


Two alternative approaches

Two Alternative Approaches


Aim and methodology

Aim and Methodology


Topics to be covered

Topics to be covered


Saccharomyces species specific

Saccharomyces species-specific


Rapid identification of yeasts

2011


Discovery of s eubayanus

Discovery of S. eubayanus*


S eubayanus species specific primers have been developed 228bp

S.eubayanus species-specific primers have been developed (228bp)


S eubayanus with hybrid strains

S. eubayanus with hybrid strains


Multiplex false positives

Multiplex False Positives


Summary

Summary


Genus specific primers

Genus-specific primers


A use alignments as for species specific

A. Use alignments (as for species-specific)


B look for regions within saccharomyces orfs that are missing in outgroups

B. Look for regions within Saccharomyces ORFS that are missing in outgroups


Sec24p structure

Sec24p structure


C use genes absent in non saccharomyces species yeast gene order browser ygob

C. Use genes absent in non-Saccharomyces speciesYeast Gene Order Browser (YGOB)


Results

Results


Conclusions

Conclusions


Zygosaccharomyces species

Zygosaccharomyces species


Species specific colony pcr

Species-specific Colony PCR


Z siamensis

Z. siamensis


Zygosaccharomyces genus specific primers

Zygosaccharomyces Genus-specific Primers


Zygosaccharomyces genus specific primers1

Zygosaccharomyces Genus-specific Primers


Conclusions for zygosaccharomyces

Conclusions for Zygosaccharomyces


Level of detection in liquid cultures

Level of detection in liquid cultures


Limitations of the method

Limitations of the Method


Overall advantages

Overall Advantages


Conclusions1

Conclusions


Acknowledgements

Acknowledgements


Identification by molecular methods

Identification by Molecular Methods


Hybrid detection in s pastorianus

Hybrid detection in S. pastorianus


Hybrid detection in s pastorianus1

Hybrid detection in S. pastorianus


Hybrid detection in s pastorianus2

Hybrid detection in S. pastorianus


Hybrid detection in s pastorianus3

Hybrid detection in S. pastorianus


Current saccharomyces species

Current Saccharomyces species


Tests against a diverse global collection s paradoxus specific primers

Tests against a diverse global collectionS. paradoxus specific primers


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