Rapid identification of yeasts
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Rapid Identification of Yeasts. Rapid Identification. Two Alternative Approaches. Aim and Methodology. Topics to be covered. Saccharomyces species-specific. 2011. Discovery of S. eubayanus*. S. eubayanus species-specific primers have been developed (228bp).

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Rapid Identification of Yeasts

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Rapid Identification of Yeasts


Rapid Identification


Two Alternative Approaches


Aim and Methodology


Topics to be covered


Saccharomyces species-specific


2011


Discovery of S. eubayanus*


S.eubayanus species-specific primers have been developed (228bp)


S. eubayanus with hybrid strains


Multiplex False Positives


Summary


Genus-specific primers


A. Use alignments (as for species-specific)


B. Look for regions within Saccharomyces ORFS that are missing in outgroups


Sec24p structure


C. Use genes absent in non-Saccharomyces speciesYeast Gene Order Browser (YGOB)


Results


Conclusions


Zygosaccharomyces species


Species-specific Colony PCR


Z. siamensis


Zygosaccharomyces Genus-specific Primers


Zygosaccharomyces Genus-specific Primers


Conclusions for Zygosaccharomyces


Level of detection in liquid cultures


Limitations of the Method


Overall Advantages


Conclusions


Acknowledgements


Identification by Molecular Methods


Hybrid detection in S. pastorianus


Hybrid detection in S. pastorianus


Hybrid detection in S. pastorianus


Hybrid detection in S. pastorianus


Current Saccharomyces species


Tests against a diverse global collectionS. paradoxus specific primers


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