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Lives of the Scientist

Lives of the Scientist. Genetic Basis of Differentiation. Events in time and space. Genetic Basis of Differentiation. Events in time and space. . . . driven by patterned gene expression. Genetic Basis of Differentiation. Events in time and space . . .

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Lives of the Scientist

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  1. Lives of the Scientist

  2. Genetic Basis of Differentiation Events in time and space . . .

  3. Genetic Basis of Differentiation Events in time and space . . . . . . driven by patterned gene expression

  4. Genetic Basis of Differentiation Events in time and space . . . . . . driven by patterned gene expression

  5. Genetic Basis of Differentiation Nostoc NH3 N2 NH3 Events in time and space . . . . . . driven by patterned gene expression

  6. Genetic Basis of Differentiation Histidine Kinase How? Environmental Signal Developmental Response NH3

  7. Genetic Basis of Differentiation P AT How? Environmental Signal Developmental Response NH3 Histidine Kinase

  8. Genetic Basis of Differentiation P P Response Regulator How? Environmental Signal Developmental Response NH3 histidine Histidine Kinase

  9. Genetic Basis of Differentiation P P Response Regulator NpR3010 ? ? ? How? Environmental Signal Developmental Response NH3 Histidine Kinase

  10. AATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTTAATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTT Genetic Basis of Differentiation P P Response Regulator NpR3010 ? ? ? How? Environmental Signal Developmental Response NH3 Histidine Kinase

  11. Histidine Kinase Nostoc punctiforme NpR3010 Anabaena PCC 7120 Trichodesmium Synechocystis PCC 6803 Conserved Genes Functionally Related to His Kinase Find similar genes . . . (13 total) Blast

  12. >npun_22dec03_Contig1_revised_geneNpR3010 MWHIQDSIITLSNHNQYLTFYKNQVKNPERFCRNVNQFDSQIDFVSCDIL ELKDGRFFEQYSKPLRLAEEIIGTVWSFRDITESQQAKEENRRIIQQEKQ LAEDRAYFTSMIFHEFRNPLNIISYSTSLLKRHSHHWSEEKKLQCLQNLQ TAVEQINQFTDEVLIIESVEAGKLQYELKPIDLNLFCREVLAEMSLYTKG ASQFLLFQNK*

  13. MWHIQDSIITLSNHNQYLTFYKNQVKNPERFCRNVNQFDSQIDFVSCDIL ELKDGRFFEQYSKPLRLAEEIIGTVWSFRDITESQQAKEENRRIIQQEKQ LAEDRAYFTSMIFHEFRNPLNIISYSTSLLKRHSHHWSEEKKLQCLQNLQ TAVEQINQFTDEVLIIESVEAGKLQYELKPIDLNLFCREVLAEMSLYTKG ASQFLLFQNK

  14. >npun_22dec03_Contig1_revised_geneNpR3008 LSPYLEACCLRISASVSYQRAAEDIEYLTGVEVSKSVQQRLVHRQNFELP QVESTVEELSVDGGNIRIRTIKGQVCDWKGYKATCLHEKQAIAASFQENS LVIDWVKSQSIAPILTCLGDGHDGIWNIVRDFAPEHQRREVLDWFHLMEN LHKIGGSNQRLNQAKILLWQGKVDDAIAVFADCQLKQAFNFCTYLEKHRH RIVNYQYYQAEQICSIGSGAIESTVKQIDRRTKISGAQWKSDNVPQVLAQ RQSLSQWINLCSLNKNWDAPMKSSVERLSDYPVAR*

  15. TRANSPOSON transposase A new family of proteins?!A type of transposase? ...ATTTCTCTAGAAAGGCTGAAGGGGGGACAAGCACCCGAAAGCCTTTGTGCT......TAAAGAGATCTTTCCGACTTCCCCCCTGTTCGTGGGCTTTCGGAAACACGA... ...ATACAGTCAGCTTTATAGGCTTCATGTCGCCCCTTCAGCTAGAAAGGTACATA......TATGTCAGTCGAAATATCCGAAGTACAGCGGGGAAGTCGATCTTTCCATGTAT...

  16. TRANSPOSON transposase A new family of proteins?!A type of transposase? ...ATTTCTCTAGAAAGGCTGAAGGGGGGACAAGCACCCGAAAGCCTTTGTGCT......TAAAGAGATCTTTCCGACTTCCCCCCTGTTCGTGGGCTTTCGGAAACACGA... ...ATACAGTCAGCTTTATAGGCTTCATGTCGCCCCTTCAGCTAGAAAGGTACATA......TATGTCAGTCGAAATATCCGAAGTACAGCGGGGAAGTCGATCTTTCCATGTAT...

  17. TRANSPOSON transposase A new family of proteins?!A type of transposase? ...ATTTCTCTAGAAAGGCTGAAGGGGGGACAAGCACCCGAAAGCCTTTGTGCT......TAAAGAGATCTTTCCGACTTCCCCCCTGTTCGTGGGCTTTCGGAAACACGA... ...ATACAGTCAGCTTTATAGGCTTCATGTCGCCCCTTCAGCTAGAAAGGTACATA......TATGTCAGTCGAAATATCCGAAGTACAGCGGGGAAGTCGATCTTTCCATGTAT...

  18. TRANSPOSON transposase A new family of proteins?!A type of transposase? Is Npr3008 a transposase?

  19. AATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTTAATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTT AATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTT AATAAAGCTTTACAAACCAAACTCTGGCTTCAATTGTGTAACCCAAGCTTTGATTCTTTCCTCTGTTAAATCGGATTGATTATCTTCATCAAGGGCAAGACCTACAAATTTACCATCACGAACAGCTTTAGACTCACTGAATTCATAACCTTCTGTAGGCCAATAGCCAACTGTTTCACCACCATTTTCTGAAATTTTTTCCTCTAGAATACCGAGGGCATCTTGAAATGTATCAGGATAACCAACCTGGTCTCCAGGAGCAAAATAAGCAACTTTTTTGCCGATGAAGTCAATGTTATCTAACTCATCATAAAAATTTTCCCAATCACTTTGCAATTCTCCAACATTCCAGGTAGGACAACCAACAACGATATAATCGTAGTTATTGAAATCACTTGGTTCAGCTTGTGAAATATCATATAAAGTTACAACACTATCACCACCAAACTCCTTCTGAATTATTTCTGATTCAGTTTGGGTATTGCCTGTTTGAGTACCAAAAAATAAACCAATATTAGACATTTTTACTCCTTTTATGTATTTGCAAAATTATTTCAATTAAAATATTTAGTAATAATTAATTGTTAGCTAGCTAATAATTAAATTTTTATTACAATCATTGTAAAAGGCATTGAAAAAGTAAATAAAAATTTTTATTCTACGTTATTTCAAAAATATTTACTTACATATACTTAACCTTTATAGTGATGTAATATACTCTAATTCCTATTTTACTTATAAATACCATCTCAGCTTAATGTAACGAATTTTTCTGTTTATCTTTAAATACAAAAAATTCAACAAAACTACAGAAAATTAATCTTAATAACACAAAACAAGTATCAATCTGTAATACAACTAAGCTTAAATAAATTAATAGAAAGCTTCATCTATCTAATAGGTTGAGAATAGTTTATGTCTAATGACATAAATTCATTCGTGTTGATTTCATTTGGGTATATTCATCTGATTTAGGATTTACTCCATTAAGTTTGTACTCATCAATGCCCGCCTGTTGGTATCCACAATTCTCATACAGTGCGCGAGCAAAGTAATCAATCGTTCGTCGCCATATCTAACTTTGAGTCAAACAAACCAGTTGGATTACCAACCCTCAACTAATCGCTTCTTTAAGGCGAGCGATCGCACATTTAACTGTTGGTTGTCACAAGAGAACTAATACTACAGCAGTATATTTAACAACTAAGGGTGGTTCAACTTTCGCTGCGACTCCTCCAACGCGCTGAAATACACAGGACTGATGCGATCGCAAACTCTTTGACTAAATTCCATACATTATCATGACCATCTCCCAAACAAACAAGTGGGTTAACCAGATGCTGACTATTAACATCCCCTGAGTTCGGAGTTGTAGGTCTATTTGACTGGTTCAAAGCGATGATGGAACGGCTTTGTTGCATGAATTAAAAAAAGACACACCATCACCTACTTCTAGGATAGACACATCAAACGTCCCACCGCCTAAGTCAAATACCAAGATAATTTCGTTAGTTTTCTTGTCAAGTCCGTAAGCGAGGGCCGCCGCCGTGGGCTAGTTGATAATTCGCAGAACTTTAATCCCGGCAATTCTACTGGCATCTTTGGTAGCCTGCCGTTGAGAGTCATTGAAATAGGCAGGGGTGGTAATTACCGCTTGCCTCACTGGTTCCCCCAGATATGTGCTGGCATCATCTATCAGCTTGCGGACTACCTCATACCATTTCACGAAAAACCTGATACACATGTAAACTCTGAAACCCTTGCTGTATCAAAGTTTTGTAATTACGAATTACGAATTACGAATTGATATCAGCCGAGATTTCTTCGGGTGAAAATTCCTTGTTCAGAGCGGGACAGTGTAGCTTGACATTGCCATTACTGTCACGTACCACTTTGTAAGTAACTTGTTTTGCCTCTTGCGTAACTTCATCATACCTGCGCCCGATGAACCGCTTCACAGAATAAAAAGTGTTTTCTGGGTTCATTACACCCTGGCGCTT

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