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Validated LC-MS determination of hepcidin in human plasma PowerPoint PPT Presentation


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Validated LC-MS determination of hepcidin in human plasma. A new method for the determination of hepcidin peptide using LC-high resolution-MS has been fully validated. The article describing the method is in preparation. LC-HR-MS chromatogram.

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Validated LC-MS determination of hepcidin in human plasma

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Validated lc ms determination of hepcidin in human plasma

Validated LC-MS determination of hepcidinin human plasma

A new method for the determination of hepcidinpeptide using LC-high resolution-MS has been fully validated. The article describing the method is in preparation.

LC-HR-MS chromatogram

Calibration Curve; Limit of quantification = 0.25nM = 0.75ng/mL

Hepcidin 0.25nM

0.6

Hepcidin

internal standard

0.4

ZOOMED

HepcidinnM

0.2

LC-HR-MS chromatogram

LC-HR-MS chromatogram

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Blank matrix

Pork plasma

Hepcidin 50nM

Hepcidin

internal standard

Hepcidin

internal standard


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