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Cyanobacterial Biodiversity

Cyanobacterial Biodiversity. T.Bhuvaneshwari, Research Scholar, NFMC. Cyanobacteria – An Introduction. Cyanobacteria - morphologically distinct group of Oxygenic Photosynthetic organisms Gram negative prokaryote - low state of cell organization

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Cyanobacterial Biodiversity

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  1. Cyanobacterial Biodiversity T.Bhuvaneshwari, Research Scholar, NFMC.

  2. Cyanobacteria – An Introduction • Cyanobacteria - morphologically distinct group of Oxygenic Photosynthetic organisms • Gram negative prokaryote - low state of cell organization • The cell lacks well defined nucleus and the DNA floats in the protoplast • Cyanobacteria exist in all known photic habitats and can thrive in extreme levels of humidity, light, salinity, temperature, availability of oxygen or cabon di oxide and solar radiation

  3. Cyanobacterial Taxonomy Botanical Approach Morphological and cytological(Bornet and Flahaut, Gomont, Geitler and desikachary) Bacteriological Approach Polyphasic Approach- physiological,Cytological, and biochemical(Stanier, waterberry and Ripka) Molecular Approach Polyphasic Approach - phenotypic and Genotypic characters(Anagnostidis and Komarek ) Identification at the Genetic level

  4. Project Objectives • Upgradation and maintanence of marine cyanobacteria in the Repository • Axenization of the Marine cyanobacteria • Identification of the strains by adapting morphological and Molecular Methods • Revalidation of the strains for their identify, lipid and fatty acid content • Supply of cultures to the needy researchers • Bioprospecting of marine cyanobacteria for Biofuel production • Screening of cultures for lipid and fatty acid profile • Optimization of physico chemical parameters for high lipid production.

  5. Aim of MY Study • To maintain the Repository of Marine cyanobacteria • To upgradate the strength of the Repository by isolating new strains • To identify the strains Morphologically • To Characterise the isolated cyanobacteria using Molecular approach such as • 16SRNA sequencing • ITS (internaltranscribed spacer region) • cpc region

  6. Repository Maintanence Marine cyanobacterial strains of the Repository were maintained in both Solid and Liquid culture. Liquid cultures were subcultured regularly at the interval of 15 days and incubated at 25±2ºC under continuous low light illumination. For long Preservation, the cultures were also maintained in Agar Slants

  7. Isolation of marine Cyanobacterium Samples are taken from different environments covering Rocky shores , Sandy shores, Saltpans, Backwater and Estuarine areas of Rameshwaram, Krusadai, Cuddalore, Vizhingam, Jalgoan, Tuticorn and Andaman They are processed immediately and pure cyanobacteria were isolated using Standard Microbiological Protocols

  8. isolated strains so far Isolate No Organism Sample Area Sample No 1 Chroococcus species Vizhingam 03 2 Gleocapsa crepidinum Gujarat 12 3 Myxosarcina burmensis Vizhingam 01 4 Aphanothece saxicola Gujarat 08 5 Aphanothece saxicola Gujarat 09 6 Oscillatoria probosideae Tuticorn 153 7 Oscillatoria species Tuticorn 34 8 Oscillatoria species Cuddalore 16 9 Phormidium species Rameshwaram 05 10 Phormidium species Krusadai 01 11 Phormidium tenue Krusadai 11 12 Nostoc calcicola Gujarat 06 Further 8 pure isolated strains yet to be identified

  9. Chroococcus species

  10. Gleocapsa crepidinum

  11. Myxosarcina burmensis

  12. Aphanothece saxicola

  13. Aphanothece saxicola

  14. Oscillatoria probosideae

  15. Oscillatoria species Oscillatoria species

  16. Oscillatoria species

  17. Phormidium tenue

  18. Phormidium species

  19. Phormidium species

  20. Nostoc calcicola

  21. Molecular Approaches

  22. Molecular work carried out so far DNA were isolated for ten organism by Xanthogenate method Among them, Pcr was carried out for four samples using the Primer 16s-1F and 16s 740 R for amplifying the regions of 16s rDNA It was then Send for sequencing the results obtained were not satisfactory as the chromatogram was not good and also the sequence matches with certain bacterial 16s rDNA sequence when performed blast analysis with the available nucleotide sequence of NCBI.

  23. Other markers to be studied • Mcy (Microcystin synthesizing gene) • Gyr B (Gyrase B) • rpo C (RNA Polymerase B) • rpo D (RNA Polymerase D)

  24. Thank ThaNK you

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