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Food and Agriculture (FA) Domain Committee. MONITORING PROGRESS REPORT 2006. 3rd DC meeting, Antalya (TR), 31 Jan – 2 Feb 2007. COST Action 871 Domain Food and Agriculture (FA). Cryopreservation of crop species in Europe. CSO approval date 27/06/2006 Entry into force 20/09/2006

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slide1

Food and Agriculture (FA) Domain Committee

MONITORING PROGRESS REPORT 2006

3rd DC meeting, Antalya (TR), 31 Jan – 2 Feb 2007

slide2

COST Action 871

Domain Food and Agriculture (FA)

Cryopreservation of crop species in Europe

CSO approval date 27/06/2006

Entry into force 20/09/2006

End date 11/12/2010

slide3

Action 871: Cryopreservation of crop species in Europe

Participating Countries : 15

Chair :BE

BG, CZ, DK, FI, FR, DE, IT, NL, PL, PT, CS, SK, ES, UK

In the pipe-line:

TR, RO, GR, LU

Non-COST:

Vavoliv, Russia

Economic Dimension: 38 Million €

Duration: 4 years

management report data part 2
Management Report data, part 2
  • Chair of the Action:Dr. Bart Panis:

Laboratory of Tropical Crop Improvement, K.U.Leuven,Kasteelpark Arenberg 13, B-3001 Leuven, BELGIUM

  • Vice chair of the Action:Professor Paul LYNCH

Biological and Forensic Sciences department,,School of Science

University of Derby, Kedleston Road

DE22 1GB Derby, United Kingdom

  • Rapporteur:Dr. Charles Spillane:

Genetics & Biotechnology Lab, Biochemistry DepartmentUniversity College Cork, Lee Maltings 2.1, Cork, Ireland

  • Science Officer:

B. STOL

  • Administrative Officer:

C. PEETERS

  • Action website: in preparation
slide5

Management Report data, part 3

OBJECTIVES

The main objective of this action is to improve and apply technologically advanced techniques for plant genetic resources conservation of crops that are grown/ and or conserved in Europe with main emphasis on long-term conservation through cryopreservation.

slide6

Management Report data, part 3

Specific objectives of CRYOPLANET ?

  • Objective 1: To screen in detail the current utilization of plant cryopreservation in Europe.
  • Objective 2: To screen and compare the efficiency of existing plant cryopreservation protocols.
  • Objective 3: To improve fundamental knowledge about cryoprotection through the determination of physico-biochemical changes associated with tolerance towards cryopreservation.
  • Objective 4: To develop new plant cryopreservation protocols.
  • Objective 5: To assure the genetic stability and true-to-typeness of plants after cryopreservation.
  • Objective 6: To apply cryopreservation to European plant germplasm collections.
  • Objective 7: To proof the environmental, social and economic impact of plant cryopreservation.
slide7

WG1

Fundamental aspects of cryopreservation/cryoprotection and genetic stability

Optimisation

Feed back

WG2

Technology, application and validation of plant cryopreservation

Management Report data, part 4

slide8

Proteins

Sugars

Water thermal

behavior

Polyamines

Membrane

components

Cytoskeletal

protein

Oxidative

stress

WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability

  • 1.1. Fundamental aspect of cryopreservation and cryoprotection
  • Elucidation of the physico-biochemical background of cryoprotection and cryopreservation.

Professor Pawel PUKACKI (chair WG1)

Physiology of Abiotic Stress LabInstitute of Dendrology, Polish Academy of SciencesParkowa 5, 62-035 KornikPoland

slide9

WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability

  • 1.2. Genetic stability and authenticity
  • Assessment of the genetic integrity of plants to determine if they are ‘true to type’ after cryopreservation.

Dr. M. Angeles REVILLA BAHILLO (co-chair WG1)Departamento Biología de Organismos y SistemasFaculty of Biologia, Universidad de OviedoCatedrático Rodrigo Uría s/n, 33071 OviedoSpain

slide10

WG2: Technology, application and validation of plant cryopreservation

  • 2.1. Technology aspects of cryopreservation
  • Applications of different cryopreservation protocols to different plant species and tissues.

Dr. Florent ENGELMANN (chair WG2)UR 141, IRDBP 64501, 911 avenue Agropolis34394 Montpellier cedex 05France

slide11

WG2: Technology, application and validation of plant cryopreservation

  • 2.2. Impact and applications of cryopreservation in plants
  • genebanks, establishment of cryo-bank and dissemination of results

Dr. Joachim KELLER (co-chair WG2)

Leibniz Institute of Plant Genetics and Crop Plant Research IPKCorrensstraße 3, 466 GaterslebenGermany

slide12

State of the art (Europe/rest world)

  • National Seed Storage Laboratory (NSSL) (Fort Collins, Colorado, USA): 2,100 accessions of apple (dormant buds)
  • National Clonal Germplasm Repository (NCGR) of Corvallis (USA): 104 accessions of pear (shoot tips);
  • International Potato Centre (CIP) (Lima, Peru) : 345 potato accessions
  • Tissue Culture BC Research Inc.(Vancouver, BC, Canada) : 5000 accessions representing 14 conifer species
  • AFOCEL (Association Forêt Cellulose) of France, with over 100 accessions of elm (dormant buds);
  • National Institute of Agrobiological Resources (NIAR) of Japan, with about 50 accessions of mulberry.
  • IRD (Montpellier, France) : 80 accessions of oil palm
  • IPK (Gatersleben, Germany)/ DSMZ (Braunschweig, Germany) : 519 old potato varieties
  • INIBAP, Laboratory of Tropical Crop Improvement, K.U.Leuven (Heverlee, Belgium) : 500 banana accessions
slide13

Why is cryopreservation not more widely applied?

  • the unavailability of efficient and robust cryopreservation protocols applicable to many plant species and diverse germplasm types
  • limited awareness of plant researchers unacquainted to recent developments in cryogenic storage methods
  • lack of coordinated research on plant cryopreservation.
  •  COST action like “CRYOPLANET” can make the difference
slide14

Time table

MC meeting: Management committee meeting; WG meeting: Working group meeting; STSMs: Short-term scientific missions; Workshop*: Timing of the Inter-COST Workshops will be defined in agreement with the Management committee of that specific Action.

meetings planned
Meetings planned

1/ WG1 meeting

April 13-14 Oviedo (Spain)

Organiser Dr. M. Angeles REVILLA BAHILLO

2/ WG2 meeting combined with meeting of executive committee May 11-12 Firenze (Italy)

Organiser Dr. Lambardi MAURIZIO

3/ Management Committee meeting

In combination with the Society for Low Temperature Biology Annual Scientific meeting at the University of Derby (12-14th Sept 2007) hosted by Professor Paul LYNCH

4/ Training Workshop on DSC and thermal analysis (8 researchers)

End 2007

Organiser Dr. Milos FALTUS (Prague, Czech Republic)

slide16

Dissemination plans

  • Articles in refereed scientific journals
  • Common reviews, books
  • A public website (information about the project, the achievements, services/consultancies offered and announcements of training workshops.
  • Information on the official webpages of the collaborating institutions.
  • The consortium will organize workshops for scientists, germplasm curators regulatory bodies and policy makers
  • At the end of the Action, the consortium will offer its expertise as a service to the EU.
  • Presentations at International Conferences, for promoting the European know-how and increasing the international collaboration.
  • Teaching activities in Universities at undergraduate and post-graduate level. Young scientists and engineers will thus be trained and informed on the latest developments in cryopreservation.

Significant need to develop dissemination plan. This will be a major agenda item at Executive Committee Meeting in May (Firenze). Members of COST 871 need to feedback ideas to the Executive Committee by end of April 2007

conclusions
Conclusions
  • Everything is in place
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