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Experimental Design in fMRI: Methods for Dummies

This book provides an overview of experimental design in fMRI, including types of design, timing parameters, and design efficiency. It also discusses the importance of correctly designing experiments and explores different types of design, such as categorical, factorial, and parametric designs.

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Experimental Design in fMRI: Methods for Dummies

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  1. Experimental Design and Efficiency in fMRI Heidi Bonnici and Sinéad Mullally Methods for Dummies 13th January 2010

  2. Overview • Experimental Design • Types of Experimental Design • Timing parameters – Blocked and Event-Related Design • Design Efficiency • Response vs Baseline (signal-processing) • Response 1 - Response 2 (statistics)

  3. Overview • Experimental Design • Types of Experimental Design • Timing parameters – Blocked and Event-Related Design • Design Efficiency • Response vs Baseline (signal-processing) • Response 1 - Response 2 (statistics)

  4. Main Take Home Point of Experimental Design Make sure you’ve chosen your analysis method and contrasts before you start your experiment

  5. Why is it so important to correctly design your experiment? • Main design goal: To test specific hypotheses • We want to manipulate the subject’s experience and behaviour in some way that is likely to produce a functionally specific neurovascular response. • What can we manipulate? • Stimulus type and properties • Stimulus timing • Subject instructions

  6. Overview • Experimental Design • Types of Experimental Design • Timing parameters – Blocked and Event-Related Design • Design Efficiency • Response vs Baseline (signal-processing) • Response 1 - Response 2 (statistics)

  7. Types of Experimental Design • Categorical – comparing the activity from one task to another task • Factorial - combining two or more factors within a task and looking at the effect of one factor on the response to other factor • Parametric – exploring systematic changes in the brain responses according to some performance attributes of task

  8. Categorical Design: Subtraction Assumption of pure insertion: One task does not affect the effect of another task. Comparing the activity of one task to another task considering the fact that the neural structures supporting cognitive and behavioural processes combine in a simple additive manner Can only test for one effect Example: Task: decide for each noun whether it refers to ananimateorinanimateobject. goat bucket

  9. Categorical Design: Conjunction A-B Tests multiple effects Does not depend on pure insertion – conjunction discounts interaction terms two or more distinct task pairs each share a common processing difference common areas of activation for each task pair Task pairs independent (AI-BI) & (AII-BII)

  10. Load task Factorial design • Combining two or more factors within a task and looking at the effect of one factor upon the other/s. MOTION NO MOTION Rees, Frith & Lavie (1997) A B C D LOW LOAD HIGH • A – Low attentional load, motion • B – Low attentional load, no motion • C – High attentional load, motion • D – High attentional load, no motion

  11. MOTION NO MOTION Terminology A B C D LOW LOAD HIGH • Simple main effects • Main effects • Interaction terms

  12. MOTION NO MOTION SIMPLE MAIN EFFECTS A B C D LOW LOAD HIGH • A – B: Simple main effect of motion (vs. no motion) in the context of low load • B – D: Simple main effect of low load (vs. high load) in the context of no motion • D – C: ? • Simple main effect of no motion (vs. motion) in the context of high load The inverse simple main effect of motion (vs. no motion) in the Context of high load OR

  13. MOTION NO MOTION MAIN EFFECTS MAIN EFFECTS A B C D • (A + B) – (C + D): • the main effect of low load (vs. high load) irrelevant of motion • Main effect of load • (A + C) – (B + D): ? • The main effect of motion (vs. no motion) irrelevant of load •  Main effect of motion LOW LOAD HIGH

  14. MOTION NO MOTION INTERACTION TERMS INTERACTION TERMS A B C D • (A - B) – (C - D): • the interaction effect of motion (vs. no motion) greater under low (vs. high) load • (B - A) – (D - C): ? • the interaction effect of no motion (vs. motion) greater under low (vs. high) load LOW LOAD HIGH

  15. MOTION NO MOTION Factorial design in SPM A B C D • How do we enter these effects in SPM? • Simple main effect of motion in the context of low load: • A vs. B or (A – B) LOW LOAD HIGH A B C D [1 -1 0 0]

  16. Factorial design in SPM A B C D • Main effect of low load: • (A + B) – (C + D) • Interaction term of motion greater under low load: • (A – B) – (C – D) [1 1 -1 -1] A B C D [1 -1 -1 1]

  17. Parametric Design exploring systematic changes in the brain responses according to some performance attributes of task • Nonlinear Polynomial expansion • Linear Cognitive components and dimensions Assumption: as the task becomes more difficult blood flow to the regions specialised for task analysis will increase

  18. Overview • Experimental Design • Types of Experimental Design • Timing parameters – Blocked and Event-Related Design • Design Efficiency • Response vs Baseline (signal-processing) • Response 1 - Response 2 (statistics)

  19. Timing Parameters – Blocked Design • It involves presenting two conditions – an activation (A) condition and a baseline (B) condition. Each condition is presented for an identical epoch of time. Task A Task B Task A Task B Task A Task B Task A Task B Task A REST Task B REST Task A REST Task B REST

  20. What baseline should you choose? • Task A vs. Task B • Example: Squeezing Right Hand vs. Left Hand • Allows you to distinguish differential activation between conditions • Does not allow identification of activity common to both tasks • Can control for uninteresting activity • Task A vs. No-task • Example: Squeezing Right Hand vs. Rest • Shows you activity associated with task • May introduce unwanted results

  21. Choosing Length of Blocks • Longer blocks allow for stability of extended patterns of brain activation. • Shorter blocks allow for more transitions between tasks. • Task-related variability increases with increasing numbers of transitions

  22. Pros and Cons of Blocked Design Pros: • Avoid rapid task-switching (e.g. patients) • Fast and easy to run; • Good signal to noise ratio Cons: • Expectation • Habituation • Signal drift • Poor choice of baseline may preclude meaningful conclusions • Many tasks cannot be conducted repeatedly

  23. Timing Parameters – Event-Related Design • It allows different trials or stimuli to be presented inarbitrary sequences. • Jittering events can reduce possibility of correlated regressors – increased efficiency • time

  24. Pros and Cons of Event-Related Design Pros: • Real world testing • Eliminate predictability of block designs (e.g. expectation); • Can look at novelty and priming; • Can look at temporal dynamics of response. Cons: • Low statistical power (small signal change) • More complex design and analysis (esp. timing and baseline issues).

  25. Overview Experimental Design Types of Experimental Design Timing parameters – Blocked and Event-Related Design Design Efficiency What is efficiency Signal Processing perspective General Advice

  26. Efficiency is… … a numerical value which reflects the ability of your design to detect the effect of interest.

  27. Efficiency is… … a numerical value which reflects the ability of your design to detect the effect of interest. • General Linear Model: • Y = X . β + ε • Data Design Matrix Parameters error • Efficiency (e) is the ability to estimate β, given the design matrix X

  28. Efficiency is… e (c, X) = inverse (σ2 cT Inverse(XTX) c) e (c, X) is specific for a given contrast (c), given the question that you are trying to answer (with your design X). So, to optimise experimental design: minimise the variance in the contrast i.e. minimise [cT (XTX)] by maximising [cT Inverse(XTX)] we assume that noise variance (σ 2) is unaffected by changes in X. All we can alter in this equation is X. Therefore we minimise the variance (a priori) to maximise efficiency: by the spacing and sequencing of epochs/events in our design matrix ensuring that your regressors are not correlated (for more details see Rik Henson’s website) Y = X β + ε The inverse of the variance within theestimated β, for this specific contrast

  29. Background: terminology Trial - replications of a condition A trial consists of one or more components, that may be: “events” or “impulses” - brief bursts of neural activity “epochs” - periods of sustained neural activity SOA (Stimulus Onset Asynchrony) - time between the onsets of components. Also referred to as the ITI (inter-trial interval). ISI (Inter-Stimulus Interval) - time between offset of one component and onset of next SOA = ISI + Stimulus Duration For events: SOA = ISI (as events are assumed to have zero duration)

  30. Signal Processing Signal processing is the analysis, interpretation, and manipulation of signals. Given that we can treat fMRI volumes as time series (for each voxel) it is useful to adopt a signal-processing perspective. Using a “linear convolution” model, the predicted fMRI series is obtained by convolving a neural function (e.g. stimulus function) was an assumed IR.

  31. The BOLD Impulse Response (IR) A BOLD response to an impulse (brief burst) of activity typically has the following characteristics: A peak occurring at 4-6s Followed by an undershoot from approximately 10-30s

  32. Fixed SOA = 16s Stimulus (“Neural”) HRF Predicted Data  = Not particularly efficient…

  33. = Fixed SOA = 4s Stimulus (“Neural”) HRF Predicted Data Very Inefficient…

  34. = Randomised, SOAmin= 4s Stimulus (“Neural”) HRF Predicted Data More Efficient, despite using only half as many stimuli as previous…

  35. = Blocked, SOAmin= 4s Stimulus (“Neural”) HRF Predicted Data but this design is even more Efficient…

  36. Background: terminology The fourier transformation decomposes a function into the sum of a (potentially infinite) number of sine wave frequency components. A frequency domain graph shows how much of the signal lies within each given frequency band over a range of frequencies Here the sine wave that best matches the basic on-off alternation has a dominant frequency corresponding to its ‘fundamental’ frequency: F0 = 1/(20s+20s) = 0.025 Hz Plus ‘harmonics’ – capture the sharper edges of the square-wave function relative to the fundamental sinusoid

  37. = Blocked, epoch = 20s Stimulus (“Neural”) HRF Predicted Data  = • A convolution in time is equivalent to a multiplication in frequency space • In this way the transformed IR acts as a filter: passes low frequencies but attenuates higher frequencies.

  38. = Blocked, epoch = 20s Stimulus (“Neural”) HRF Predicted Data  = Efficient design as most of the signal is ‘passed’ by the IR filter

  39. So what is the most efficiency design of all…

  40. Sinusoidal modulation, f = 1/33s Stimulus (“Neural”) HRF Predicted Data  = The most efficient design of all!

  41. Highpass Filtering fMRI noise tends to have two components: Low frequency ‘1/f’ noise e.g. physical (scanner drifts); physiological [cardiac (~1 Hz), respiratory (~0.25 Hz)] Background white noise Highpass filters aims to maximise the loss of noise but minimise the loss of signal. We apply the highpass filter to the lowpass filter inherent in the IR to creast a single ‘band-pass’ filter (or ‘effective HRF’).

  42. = “Effective HRF” (after highpass filtering) (Josephs & Henson, 1999) Blocked (80s), SOAmin=4s, highpass filter = 1/120s Stimulus (“Neural”) HRF Predicted Data  = Don’t have long (>60s) blocks!

  43. =  = Randomised, SOAmin=4s, highpass filter = 1/120s Stimulus (“Neural”) HRF Predicted Data (Randomised design spreads power over frequencies)

  44. General Advice (Rik Henson) Scan for as long as possible (as increasing the number of volumes increasing the degrees of freedom). For group studies increasing the number of participants adds more statistical power that increasing the number of DF. Do not contrast conditions that are far apart in time (because of low-frequency noise in the data). Randomize the order, or randomize the SOA, of conditions that are close in time. http://www.mrc-cbu.cam.ac.uk/Imaging/Common/fMRI-efficiency.shtml

  45. Conclusions: Blocked designs generally most efficient (with short SOAs, given optimal block length is not exceeded) However, psychological efficiency often dictates intermixed designs, and often also sets limits on SOAs With randomised designs, optimal SOA for differential effect (A-B) is minimal SOA (>2 seconds, and assuming no saturation), whereas optimal SOA for main effect (A+B) is 16-20s Inclusion of null events improves efficiency for main effect at short SOAs (at cost of efficiency for differential effects) If order constrained, intermediate SOAs (5-20s) can be optimal If SOA constrained, pseudorandomised designs can be optimal (but may introduce context-sensitivity) Remember an optimal design for one contrast may not be optimal for another http://www.mrc-cbu.cam.ac.uk/Imaging/Common/fMRI-efficiency.shtml

  46. Useful links and thanks • Antoinette Nicolle • http://imaging.mrc-cbu.cam.ac.uk/imaging/DesignEfficiency • Nick and Edoardo’s slides from MfD 2008

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