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BioWire Progress Report Week Four

BioWire Progress Report Week Four. Orr Ashenberg, Patrick Bradley, Connie Cheng, Kang-Xing Jin, Danny Popper, Sasha Rush. Photolithography Process. Design a CAD image of the shapes we want to make. Our Mask. Our Mask with labels. Photolithography Process.

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BioWire Progress Report Week Four

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  1. BioWire Progress ReportWeek Four Orr Ashenberg, Patrick Bradley, Connie Cheng, Kang-Xing Jin, Danny Popper, Sasha Rush

  2. Photolithography Process • Design a CAD image of the shapes we want to make.

  3. Our Mask

  4. Our Mask with labels

  5. Photolithography Process • Design a CAD image of the shapes we want to make. • Dr. Lu makes the CAD into a mask. • He estimates 30 minutes ($50)

  6. Photolithography Process • Design a CAD image of the shapes we want to make. • Dr. Lu makes the CAD into a mask. • He estimates 30 minutes ($50) • Someone from Kit’s lab makes the mask into a positive master mold in the cleanroom.

  7. Photolithography Process • Make a negative PDMS from the master. • Make positive agar stamps from the PDMS negative. • Pour cell culture onto stamp and let sit for 24 hours. • (Optional) Stamp down a perimeter of cells around the stamping area and grow for 24 hours. • Stamp the cells onto the agar plate.

  8. Progress • Design has been approved by Dr. Lu. • High gel strength agar will arrive later today. • Find someone from Kit’s lab to make the master. • Get an XY motor for visualization. • Get an XYZ motor for possible use in precise stamping for the 50m stamp (150m depth).

  9. Future Designs • Different feature sizes. • Once line signaling works, move to shapes. • Possibility of controlling cell density by making lines out of small checkerboards.

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