BioWire Progress Report Week Five

1. BioWire Progress ReportWeek Five Orr Ashenberg, Patrick Bradley, Connie Cheng, Kang-Xing Jin, Danny Popper, Sasha Rush

2. Continued building Las, RhI circuits and Lux receiver test constructs Reorganized BioBrick information database Submitted mask order to Dr. Lu Ordered CFP filter Last Week

3. Building the Circuits • Sasha created a REALLY HOT DATABASE OF LIGATIONS OMFG HOT • Higher success rate with transformations - now within 2 cycles of finishing all of our parts

4. Photolithography • They did… stuff. Hottt.

5. Continuous Model • It’s being done?

6. This Week • Parts scheduled to be finished today • J06004 - Receiver with Repressor Component • J06005 - Weiss Receiver Construct • J06007,8 - BioWire Receiver Constructs with propagation! • Conduct experiments on Sender Reporter Test Construct with CFP filter • Plan and conduct experiments on Receiver Constructs • Finish building lux, las constructs

7. Updated Schedule • Week 1 (6/6): Project Choice and Design • Week 2 (6/13): Got parts and set up tests • Week 3 (6/20): Began building test constructs, finished sender • Week 4 (6/27): Finish receiver, receiver w/repressor; CAD a mask • Week 5 (7/4): Continued building parts, ordered mask • Week 6 (7/11): Test constructs, finish building parts, get master mold, make PDMS molds • Week 7 (7/18): Construct IFFL • Week 8 (7/25): Test it • Week 9 (8/1): “ • Week 10 (8/8): “ • Week 11 (8/15): “ • Week 12 (8/22): “