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Typhoid Diagnostics for Measuring Disease Burden

Typhoid Diagnostics for Measuring Disease Burden. Fondation Mérieux developed a molecular assay to detect Typhoid 1st phase ended Dec 2013 Excellent results obtained with samples from CHRF-Bangladesh Improved senstitivity over hemoculture reference method

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Typhoid Diagnostics for Measuring Disease Burden

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  1. Typhoid Diagnostics for Measuring Disease Burden • Fondation Mérieux developed a molecular assay to detect Typhoid • 1st phase ended Dec 2013 • Excellent results obtained with samples from CHRF-Bangladesh • Improved senstitivity over hemoculture reference method • Bill & Melinda Gates Foundation funded research • 2nd phase until July 2014 • Validation in a large number of clinical samples from Bangladesh • New multicenter project in preparation • Large Multicentre research study including some GABRIEL member labs • Head to Head comparaison of LPE assay with other potential typhoid diagnostic assays

  2. Typhoid Diagnostics for Measuring Disease Burden

  3. Typhoid Diagnostics for Measuring Disease Burden Turn-around-time (TAT) Blood culture: 24-48 Hours 18-24 Hours Blood culture Identification Total TAT 42-72h PCR assay: 5 Hours 3 Hours Pre enrichment step Extraction + PCR Total TAT 8h

  4. Typhoid Lab Diagnostics FTD Enteric Fever kit

  5. Perspectives: Typhoid Diagnostics for Measuring Disease Burden • Extended study • Evaluate lower volume of blood: 3ml vs. 5ml • Need to evaluate the discrepant results • 2 sites in Bangladesh: CHRF for pediatric samples and ICDDR,b for adult samples • New typhoid multicenter study • At least 2 countries in Asia and 2 countries in Africa • Possibility of the involvement of the GABRIEL network laboratories

  6. Bangladesh, Urban and Rural Populations, 1950-2050 UNDP 2009

  7. Emerging Infectious Diseases:Global Trends and Hot Spots Zoonotic wildlife Zoonotic non-wildlife Multi drug resistance pathogens Vector borne pathogen Jones et al. Nature Feb 21. 2008

  8. Death Due to Respiratory Infections

  9. Diarrheal Deaths

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