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Chromatography

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Chromatography

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    1. 1 Chromatography Porous Carbon Monolithic Columns for Chromatographic Applications Wednesday 27 December, 2010

    2. 2 Massive structure such as a mountain, consisting of a single piece or material WHAT IS MONOLITH?

    3. 3 CARBON MONOLITHS: PROPERTIES AND Low backpressure Alternative separation selectivity to silica and polymer-based monoliths High chemical and mechanical stability in alkaline and acidic eluents and organic solvents Relatively high surface area APPLICATIONS Adsorption of Large Molecules Catalytic Support in Fuel Cells Energy Storage in Double-Layer Capacitors Chromatographic Applications

    4. 4 PREPARATION OF CARBON MONOLITHIC COLUMNS

    5. 5 CHARACTERISATION OF CARBON MONOLITHIS Scanning Electron Microscopy (SEM)

    6. 6 BET MEASUREMENTS OF CARBON MONOLITHIS

    7. 7 MERCURY POROSIMETRY

    8. 8 BACKPRESSURE DETERMINATION

    9. 9 NP–HPLC PRELIMINARY SEPARATIONS CARBON MONOLITHIC COLUMN Preliminary separation of mixture of butylbenzene – acetophenone on the 5 µm glassy carbon monolithic column (30 x 6.0 mm) Mobile phase 100% Hexane Flow rate 1 ml/min Backpressure 8 bar UV detection 254 nm Injection volume 15 µL

    10. 10 CONCLUSIONS Carbon monolithic columns with minimal silica residue were successfully prepared The carbon monoliths were characterised using different techniques such as SEM, BET, mercury porosimetry & backpressure determination The pyrolysed carbon had a total surface area of 176 m2/g (1 µm), 154 m2/g (2 µm), 85 m2/g (5 µm) & 124 m2/g (10 µm) The carbon monolithic column investigated for normal-phase high performance liquid chromatography showing promising separation

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